Neurite Outgrowth/Degeneration Assay Service

Neurite outgrowth is a critical process in neuronal development, regeneration, and degeneration, directly reflecting neuronal function and drug effects. Our services leverage a high-content imaging platform with advanced algorithmic analysis, enabling the analysis of thousands of neurons per experiment with sub-micron precision. We provide high-precision, high-throughput in vitro detection solutions for the study of central nervous system diseases such as stroke, Alzheimer's disease (AD), Parkinson's disease (PD), etc., assisting in drug screening, toxicity assessment, and mechanism analysis. For further information regarding the products and services provided, project-specific consultation, and pricing, please submit an inquiry here.

Overview of Neurite Outgrowth

Neurite outgrowth is a key step in the formation of protrusions, including axons and dendrites, by neurons during development. This extension occurs through complex interactions with external signals, such as neurotrophins and guidance molecules. This process requires intricate regulation of intracellular signals, including the dynamic reorganization of the cytoskeleton, involving both tubulin polymerization and actin remodeling within the growth cone, and the expansion of the cell membrane. Neurite outgrowth is a core process for the formation and maintenance of nervous system function, particularly synaptic connectivity and plasticity. Its study is of great significance for understanding neurological diseases, developing new therapies, and evaluating drug toxicity.

Our Neurite Outgrowth Assays

Fully Automated High-Content Imaging and Analysis System

Utilizing 96/384-well plates for neuronal culture, combined with fluorescent antibody markers (e.g., β-Tubulin, MAP2), our system captures high-resolution neuronal network images using a confocal microscope, capable of detecting up to 4 fluorophores. Our advanced analysis software, equipped with sophisticated algorithms for automated neurite tracing, segmentation, and growth cone analysis, enables accurate quantification of parameters such as the number, length, branch points, and growth cone dynamics of neurites.

Multi-Dimensional Analysis

Multi-dimensional analysis is supported, including two-dimensional and three-dimensional neurite growth analysis, performed using confocal z-stack imaging. This enables quantitative evaluation of parameters such as neurite density and branching complexity, providing comprehensive data output in CSV and graphical formats.

Toxicity and Regeneration Analysis

Toxicity and regeneration research utilizing neurite growth experiments is crucial for evaluating the toxic effects of compounds on neurons or their ability to promote neural regeneration. This system is widely used in drug development for AD, PD, and other neurodegenerative diseases. We are able to analyze primary neurons, stem cell-derived neurons, and cell lines, providing flexibility for diverse experimental needs.

Service Process

	Cell Culture and Processing
	Select appropriate cell types (e.g., primary neurons, iPSC-derived neurons) according to customer needs. Culture and process cells under specific, optimized conditions to ensure viability and consistent growth.
	Adding Stimulatory Factors or Toxins
	Precisely introduce growth factors (e.g., NGF, BDNF) or toxins (e.g., glutamate, beta-amyloid) using microfluidic devices or culture plates. Various drug concentrations, and time points are available.
	Image Acquisition and Analysis
	Use high-resolution microscopes and automated image analysis software to quantitatively analyze neurite morphology.
	Data Analysis and Reporting
	Provide detailed experimental data and graphs, including indicators such as neurite length, density, and number of branches, and generate professional reports for client reference.