- NeuroMab™ Mouse Anti-LRP1 Monoclonal Antibody (CBP3363) (Cat#: NAB-0720-Z6479)
- NeuroMab™ Anti-FGFR1 Antibody,Clone NR3547P (Cat#: NRP-0422-P1244)
- Mouse Anti-Human α-Synuclein Phospho (Tyr39) (CBP3706) (Cat#: NAB201250LS)
- NeuroMab™ Anti-GD2 Antibody,Clone NR3007P (Cat#: NRZP-1222-ZP767)
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- NeuroMab™ Anti-Tau Antibody,Clone NR2218P (Cat#: NRP-0422-P2275)
- NeuroMab™ Mouse Anti-EFNB2 Monoclonal Antibody (CBP1159) (Cat#: NAB-0720-Z4396)
- NeuroMab™ Anti-CD32b Antibody, Clone NR130P (Cat#: NRP-0422-P1803)
- NeuroMab™ Anti-Tau Antibody,Clone NR3320P (Cat#: NRP-0422-P1760)
- NeuroMab™ Anti-ApoC3 BBB Shuttle Antibody,Clone NR1616P (Cat#: NRZP-1022-ZP3505)
- Human Glioblastoma Cell Line SF126 (Cat#: NCL-2108P35)
- Human Astrocytes (Cat#: NCC20-9PZ01)
- iNeu™ Retinal Pigment Epithelial Cells (RPE) (Cat#: NRZP-0323-ZP92)
- Rat Schwann Cells RSC96, Immortalized (Cat#: NCL-2108P21)
- Mouse Glioma Cell Line GL261 (Cat#: NCL-2108P28)
- Rat Olfactory Ensheathing Cells (Cat#: NRZP-1122-ZP162)
- iNeu™ Human Motor Neurons (Cat#: NCL-2103-P71)
- Human Microglia Cell Line HMC3, Immortalized (Cat#: NCL-2108P38)
- iNeu™ Human Sensory Neurons (Cat#: NCL-2103-P62)
- iNeu™ Human Midbrain Dopaminergic Neurons (Cat#: NCL-21P6-003)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Amyloid beta 1-42 Kit (Cat#: NRP-0322-P2170)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Beta Amyloid (1-40), Aggregation Kit, TTF Assay (Cat#: NRZP-0323-ZP199)
- rAAV-CAG-DIO-G-Flamp1 (Cat#: NRZP-0722-ZP719)
- AAV2/2Retro-CAG-DIO-EGFP-2A-TetTox-pA [Neural Tracing] (Cat#: NTA-2012-ZP303)
- Dextran, Cy5 Labeled, 2000 kDa (Cat#: NRZP-0722-ZP22)
- pAAV-hSyn-DIO-XCaMP-R-WPRE (Cat#: NTA-2012AD-P508)
- pAAV-syn-FLEX-jGCaMP8m-WPRE (Cat#: NTA-2106-P065)
- AAV2/9-hSyn-Flpo-EGFP-WPRE-pA (Cat#: NTA-2012-ZP149)
- VSV-eGFP (Cat#: NTA-2011-ZP20)
- pAAV-EF1a-DIO-EGFP-WPRE (Cat#: NTA-2012AD-P285)
- rAAV-E-SARE-Cre-ERT2-PEST-WPRE-hGH polyA (Cat#: NTA-2010-TT342)
- AAV2 Full Capsids, Reference Standards (Cat#: NTC2101070CR)
- Lenti of Human TAR DNA binding protein (TARDBP) (NM_007375) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0832)
- Rat Parkinson disease (autosomal recessive, juvenile) 2, parkin (Park2) (NM_020093) ORF clone/lentiviral particle, Myc-DDK Tagged (Cat#: NEP-0621-R0041)
- Human huntingtin-associated protein 1 (HAP1) transcript variant 2 (NM_177977) ORF clone, Myc-DDK Tagged (Cat#: NEP-0521-R0676)
- Human superoxide dismutase 3, extracellular (SOD3) (NM_003102) ORF clone, Untagged (Cat#: NEP-0521-R0808)
- App Rat amyloid beta (A4) precursor protein (App)(NM_019288) ORF clone, Untagged (Cat#: NEP-0421-R0053)
- Mouse SOD1 shRNA Silencing Adenovirus (Cat#: NV-2106-P14)
- Human presenilin 1 (PSEN1), transcript variant 2 (NM_007318) ORF clone, TurboGFP Tagged (Cat#: NEP-0421-R0140)
- Human superoxide dismutase 1, soluble (SOD1) (NM_000454) ORF clone, TurboGFP Tagged (Cat#: NEP-0521-R0748)
- Lenti of Mouse synuclein, alpha (Snca) transcript variant (NM_001042451) ORF clone, mGFP Tagged (Cat#: NEP-0521-R0864)
- Tau Antisense Oligonucleotide (IONIS-MAPTRx) (Cat#: NV-2106-P29)
- NeuroBiologics™ Mouse Cerebrospinal Fluid (Cat#: NRZP-0822-ZP497)
- NeuroBiologics™ Monkey Cerebrospinal Fluid (Cat#: NRZP-0822-ZP495)
- NeuroBiologics™ Rat Cerebrospinal Fluid (Cat#: NRZP-0822-ZP496)
- NeuroBiologics™ Human Cerebrospinal Fluid (Cat#: NRZP-0822-ZP491)
- NeuroBiologics™ Pig Cerebrospinal Fluid (Cat#: NRZP-0822-ZP498)
- NeuroPro™ Anti-GDNF BBB Shuttle Protein, cTfRMAb-GDNF (Cat#: NRZP-0423-ZP500)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein, cTfRMAb-IDUA (Cat#: NRZP-0423-ZP498)
- NeuroPro™ Anti-NAGLU BBB Shuttle Protein, HIRMab-NAGLU (Cat#: NRZP-0423-ZP506)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein, cTfRMAb-TNFR (Cat#: NRZP-0423-ZP501)
- NeuroPro™ Anti-ASA BBB Shuttle Protein, HIRMab-ASA (Cat#: NRZP-0423-ZP504)
- NeuroPro™ Anti-Erythropoietin BBB Shuttle Protein, cTfRMAb-EPO (Cat#: NRZP-0423-ZP499)
- NeuroPro™ Anti-IDUA BBB Shuttle Protein, HIRMab-IDUA (Cat#: NRZP-0423-ZP502)
- NeuroPro™ Anti-SGSH BBB Shuttle Protein, HIRMab-SGSH (Cat#: NRZP-0423-ZP505)
- NeuroPro™ Anti-TNFR BBB Shuttle Protein, HIRMab-TNFR (Cat#: NRZP-0423-ZP510)
- NeuroPro™ Anti-EPO BBB Shuttle Protein, HIRMab-EPO (Cat#: NRZP-0423-ZP508)
Nucleo-cytoplasmic Transport Assay
In both normal and malignant tissues, specific proteins shuttling from the nucleus are essential for the regulation of the cell cycle and proliferation. Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disease affecting the motor neurons and resulting in paralysis and ultimately in death, within 2-5 years on average. Many studies have shown a connection between ALS and impairments in the nucleocytoplasmic pathway. Creative Biolabs is flexible to meet the unique needs of client neurology projects. We can apply our considerable experience with nucleo-cytoplasmic transport assay to develop the necessary analytics specific to your project.
Overview of Nucleo-cytoplasmic Transport (NCT)
NCT refers to the import and export of large molecules from the cell nucleus. Nucleocytoplasmic exchange of proteins and RNAs is mediated by receptors, guiding their cargo through nuclear pores. The peptide locator signal on each cargo determines the receptor with which it will interact. So far, three types of transporters have been identified. The best-studied group is the import protein-β-like protein family (import and export proteins, also known as nuclides). The second class comprises the small homodimeric nuclear transport factor 2 (NTF2)/p10, which imports the small GTPase Ran into the nucleus. The final group is the Tap/Mex67 family, which is involved in mRNA transport.
Fig.1 A model for PABP NCT. (Burgess & Gray, 2012)
General Workflow of NCT at Creative Biolabs
This workflow describes a new and very sensitive assay to evaluate and quantify NCT dysfunction in real-time. The import rate of NLS-NES-GFP protein (shuttle-GFP) can be quantified in real-time by fluorescence microscopy. This is done using an exportin inhibitor, thus allowing the shuttle-GFP only to enter the nucleus.
Fig.2 General workflow of NCT.
Impaired NCT is One Fundamental Pathogenesis of Neurodegenerative Diseases (NDs)
Accumulating evidence indicates that alterations of NCT are the fundamental pathological factors underlying these NDs. Under normal NCT conditions, the protein transport mechanisms enable each protein cargo to reach and retain in appropriate compartments, nucleoplasm, or cytoplasm. In a healthy neuron, most of the mRNAs should be exported to the cytoplasm for protein synthesis. Dysregulations of the protein NCT process will interfere with the normal distributions of protein cargos, leading to protein mislocalization. Protein mislocalization may occur in the nucleus or cytoplasm in diseased or aged neurons. If nuclear transcript export mechanisms are impaired in a neuron, the normal distribution of RNAs will be disrupted, such as nuclear RNA accumulation and mRNA mislocalization. Neurons are specialized cellular subtypes that possess unique features and functions, these features make neurons more vulnerable to the impairment of intracellular transport. The implication of defective NCT in NDs has been revealed by a variety of research model systems.
NCT Assay Services at Creative Biolabs
Nuclear import and export are a highly-coordinated process involving many proteins and large complexes working together on the nuclear membrane. Creative Biolabs provides flexible, robust, facile, and highly amenable to academic scale research assays with the potential to be employed in novel drug-screening applications, in particular, when combined with biochemical assays.
Creative Biolabs is 100% dedicated to the neurology research industry. Our team of highly qualified and experienced technical staff will work with you to develop and deliver assay solutions to add value to your product or project. Please feel free to contact us to discuss your needs.
Reference
- Burgess, H.M.; Gray, N.K. An integrated model for the nucleo-cytoplasmic transport of cytoplasmic poly (A)-binding proteins. Communicative & integrative biology. 2012, 5(3): 243-247.