Tau Aggregation Assay Service

The in vitro tau aggregation assay can simulate the process of tau protein misfolding and aggregation in vivo, providing an important tool for studying the role of tau protein in disease. For further information regarding the products and services provided, project-specific consultation, and pricing, please submit an inquiry here.

Introduction

Tau Aggregation Assays at Creative Biolabs

• In Vitro Aggregation Assays

In vitro aggregation experiments are an important method for studying tau protein aggregation, including experiments using recombinant or synthetic tau proteins. These methods typically use fluorescent probes (such as thioflavin T or ThT) to monitor the aggregation process of tau protein in real time. By monitoring changes in ThT fluorescence intensity, the aggregation rate and kinetic properties of tau protein can be evaluated in real time.

Fig.1 A neuronal seed model of tau accumulation.^{2, 4}

• Cell Models

Recent progress in the study of tau protein aggregation, based on cell models, is significant. Our human induced pluripotent stem cell (iPSC) platform can help you use iPSC-derived neuronal models to express fluorescently labeled tau protein through lentiviral transfection, and observe the aggregation process of tau protein in cells.

Fig.2 Modeling tau aggregation using human iPSC-derived neurons.^{3, 4}

• High Throughput Screening

To screen small molecules capable of regulating tau protein aggregation, our researchers have developed a high-throughput screening platform. This platform typically integrates fluorescent probes, automated equipment, and image analysis algorithms to efficiently evaluate the effects of compounds on tau aggregation. Linear regression kinetic analysis is used to monitor the tau aggregation rate in real time and accurately quantify the early aggregation kinetic parameters.

Fig.3 High throughput screening platform.

Applications

• Drug Development: Screening small molecules/antibodies that inhibit abnormal tau aggregation and evaluating dual regulation of compounds on oligomer toxicity and microtubule stability.
• Diagnostic Marker Development: Development of detection kits based on tau oligomers/phosphorylation sites to aid in early screening and efficacy monitoring.
• Disease Mechanism Research: Analyze the transsynaptic transmission mechanism of tau and explore the molecular drivers of neurodegenerative diseases.

To discuss pricing and scheduling for your tau research, please contact us.

References

1. Zheng, Huiting, et al. "The Enigma of Tau Protein Aggregation: Mechanistic Insights and Future Challenges." International Journal of Molecular Sciences 25.9 (2024): 4969.
2. Amorim, Ines S., et al. "A seeding-based neuronal model of tau aggregation for use in drug discovery." PLoS One 18.4 (2023): e0283941.
3. Verheyen, An, et al. "Using human iPSC-derived neurons to model TAU aggregation." PloS one 10.12 (2015): e0146127.
4. Distributed under Open Access license CC BY 4.0, without modification.