Nitric Oxide Production Assay Service

Nitric oxide (NO) is an essential neurotransmitter and signaling molecule, widely involved in physiological processes such as neurotransmission, vasodilation, and immune modulation. In multiple sclerosis (MS), NO plays a complex role, potentially exacerbating the disease through neurotoxicity while also providing protection by regulating immune and inflammatory responses. Our in vitro NO assay services, characterized by their efficiency, accuracy, and cost-effectiveness, are designed to meet the diverse needs of our clients. For further information regarding the products and services provided, project-specific consultation, and pricing, please submit an inquiry here.

NO in Multiple Sclerosis (MS)

Studies have demonstrated significantly elevated NO concentrations in the serum and cerebrospinal fluid of patients with MS. Furthermore, the expression of NO within the central nervous system (CNS) is closely correlated with disease progression. Notably, oligodendrocytes and motor neurons exhibit differential sensitivities to NO, with motor neurons displaying markedly higher sensitivity compared to oligodendrocytes. This disparity may influence the pathological mechanisms of MS, particularly the sequential events of axonal damage and myelin loss.

Nitric Oxide Quantitation Assays

• Comprehensive NO Analysis for Diverse Research Needs

Our advanced NO detection services employ a multi-faceted approach, providing precise and reliable data for a wide range of applications.

Chemical Colorimetry

Leveraging the colorimetric reaction of NO metabolites (NO₂^-/NO₃^-), this method is versatile and compatible with various sample types, including cells, tissues, and serum. Achieves a detection limit in the μM range, enabling high-throughput screening for efficient analysis.

Enzyme Activity Analysis

Accurately assesses nitric oxide synthase (NOS) activity, providing critical insights into NO synthesis capacity in neural, endothelial, and other cell types.

Customized Integrated Analysis

Offers tailored solutions by combining NO detection with complementary techniques such as Western blot and ELISA. Enables joint analysis of NO pathway proteins (e.g., eNOS, iNOS) and oxidative stress markers (e.g., nitrotyrosine), providing a comprehensive understanding of NO signaling.

• Comprehensive End-to-End Support

Sample Pre-treatment Guidelines

Provision of standardized operational protocols for cell lysis, tissue homogenization, and other procedures to prevent sample degradation.

Data Interpretation Services

In-depth analysis of correlations between NO levels and disease phenotypes, leveraging comprehensive literature databases.

Case Study: NO production during the cell suspension subculture cycle

Fig.1 NO production within subcultivation period of Col-0 and ein2-1 cultures.^{2, 3}

Empower your research with our streamlined in vitro nitric oxide testing services. We deliver efficient, accurate, and cost-effective solutions tailored to meet the diverse needs of our clientele. From fundamental research to cutting-edge drug development, our team provides expert technical support and unparalleled service quality.

Explore our comprehensive offerings and discover how we can accelerate your projects. Visit our website or contact us directly to learn more.

References

1. Yuste, Jose Enrique, et al. "Implications of glial nitric oxide in neurodegenerative diseases." Frontiers in cellular neuroscience 9 (2015): 322.
2. Novikova, Galina V., et al. "Nitric oxide has a concentration-dependent effect on the cell cycle acting via EIN2 in Arabidopsis thaliana cultured cells." Frontiers in Physiology 8 (2017): 142.
3. Distributed under Open Access license CC BY 4.0, without modification.