Service

Myelination, Demyelination and Remyelination Assay

Myelination, the process by which myelin sheaths are formed around axons in the central nervous system (CNS) and peripheral nervous system (PNS), plays a crucial role in proper neuronal function. Demyelination, on the other hand, refers to the loss or damage of these myelin sheaths. Both myelination and demyelination processes have significant implications in various neurological disorders, such as multiple sclerosis (MS). Therefore, the study of myelination, demyelination, and remyelination has gained immense importance in neuroscience research.

Creative Biolabs offers a comprehensive range of services for studying myelination, demyelination, and remyelination assays. Our team of highly skilled experts, with over 20 years of experience in the field, is dedicated to providing cutting-edge solutions to advance our understanding of these processes and develop potential therapeutics.

Assay Models

Creative Biolabs offers a range of assay models to simulate myelination, demyelination, and remyelination processes in vitro. These models include:

Primary Cell Culture Models: Primary oligodendrocyte cultures, co-cultures of oligodendrocytes and neurons, and 3D brain organoids culture systems can be utilized to study myelination and the effects of various compounds on myelin formation and maintenance.
Ex vivo Rodent Model: Organotypic cultures allow to perform myelination studies in a more complex and anatomically preserved environment; the slices can be isolated from transgenic mice for fate-mapping studies, demyelinated with toxicants and used to test the remyelinating potential of candidate compounds.
Human Induced Pluripotent Stem Cell (iPSC)-Derived Models: Human iPSC-derived oligodendrocyte models provide a valuable tool for studying human-specific aspects of myelination, demyelination, and remyelination. These models allow for the assessment of patient-specific responses and the screening of potential drug candidates.

Figure 1. In vitro models to study myelination, demyelination and remyelination. (Marangon, 2021)

Workflow for Modeling Human iPSC-Derived Oligodendrocytes

Creative Biolabs has developed a robust workflow for modeling human iPSC-derived oligodendrocytes (iOligo™), enabling the investigation of myelination in a human-relevant system. This workflow includes the following steps:

iPSC Reprogramming

Human iPSCs are generated from patient-derived somatic cells using reprogramming techniques, such as viral transduction or non-integrating methods.

Oligodendrocyte Differentiation

iPSCs are differentiated into oligodendrocyte progenitor cells (OPCs) using specific growth factors and culture conditions.

Myelination Induction

OPCs are co-cultured with neurons or axonal tracts to induce myelination. Various factors and compounds can be added to assess their effects on myelination.

Demyelination and Remyelination Assay

The remyelination potential of compounds or candidate therapeutics can be evaluated by measuring the extent of remyelination using histological and molecular analyses.

Highlights

Versatile Assay Models

We offer a range of in vitro assay models, allowing you to choose the most appropriate model for your research needs.

Timely Delivery

We are committed to delivering results in a timely manner, allowing researchers to advance their projects efficiently.

Customization

Our experienced scientists can tailor the assay protocols and models to suit your specific requirements, ensuring the best fit for your project.

Expertise

Our team of highly skilled experts possesses extensive experience in the field of neuroscience research, ensuring the highest quality of service and reliable results.

Options for Using Our iOligo™

Creative Biolabs provides flexible options for utilizing our iOligo™:

Order iOligo products for use in your lab
If you are interested in ordering iOligo and perform the assay in your lab, we offer a convenient and flexible supply of iOligo, along with a ready-to-use iOligo kit.

Cat.	Product Name
NCL-2103-P49	iNeu™ Human Oligodendrocyte Progenitor Cells (OPCs)
NCL-21P6-105	Oligodendrocyte Precursor Cell Growth Medium
NCL-21P6-106	Oligodendrocyte Precursor Cell Differentiation Medium

Outsource your experiments to us
We offer a swift and dependable service that involves collaborative experiment design to address your specific inquiries, delivering results within a span of 2 months. Our tailored projects are conducted within our cutting-edge facilities.

For further information or to initiate a discussion regarding your unique requirements, please contact us today to explore the possibilities of collaborating with our organization.

Reference

Marangon, Davide, et al. "Novel in vitro experimental approaches to study myelination and remyelination in the central nervous system." Frontiers in Cellular Neuroscience 15 (2021): 748849.

For Research Use Only. Not For Clinical Use.