Myelination, Demyelination and Remyelination Assay Service

Myelin is a key component of the myelin sheath, facilitating efficient neuron communication and enhancing nerve signal transmission speed. Demyelination occurs in certain neurological diseases, such as multiple sclerosis (MS), Guillain-Barré syndrome (GBS), and peroneal muscular atrophy (CMT).

Creative Biolabs provides extensive research services in myelination, demyelination, and remyelination assays.

Case Study

• Case Study 1

An in vitro culture system capable of replicating myelination holds promise for advancing our comprehension of myelination and demyelination mechanisms, while also uncovering potential therapeutic strategies for the treatment of demyelinating diseases. Our team of neuroscientists has successfully developed a stable and reliable in vitro culture model that can observe myelin sheath formation within 26 days. This assay involves the co-culturing of neurons and oligodendrocytes (OPC) in a specialized medium designed to promote myelination.

Fig.1: Co-culture neurons and OPCs using a medium that promotes myelination. On day 7, 16, and 26, MBP and TUJ1 staining images were obtained using a Zeiss LSM 980 confocal microscope, and graphs were generated with GraphPad Prism.

• Case Study 2

The demyelination of the axons subsequently leads to degeneration of the nerves and impaired neuronal function, contributing to the observed neuropathology. Our team has devised an in-house assay enabling the assessment of myelination and remyelination utilizing primary neurons. This assay serves as a valuable tool for the in-depth investigation and evaluation of compounds targeted at treating these conditions and related neuropathologies.

Fig.2: Primary rat spinal cultures were prepared and maintained in myelination media for > 30 days, followed by treatments as indicated in the experimental scheme. Image quantification was carried out using customized software.

Available Models

Creative Biolabs provides a broad range of assay models that replicate myelination, demyelination, and remyelination processes in vitro.

• Primary Cell Culture Models: Primary oligodendrocyte cultures, co-cultures of oligodendrocytes and neurons, and ex vivo brain slices can be used to evaluate myelination and the effects of various compounds on demyelination and remyelination. Various models are readily available and budget friendly.
• Human iPSC Derived Neuronal Cell Culture Models: Human iPSC-derived oligodendrocyte models are valuable tools for studying human-specific myelination, demyelination, and remyelination. These models can assess patient-specific responses and screen potential drug candidates, improving high-throughput readouts while ensuring each readout is accurate.
• Custom cell model: Developing your own disease model for specific research objectives.

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We provide rapid and trustworthy service that involves collaborative experimental design to meet your specific inquiries and provide results within 2 months. Our custom projects are performed in our state-of-the-art facility. For more information or to discuss your unique needs, contact us directly to explore the possibility of working with our organization.