APP Processing Assay Service

As a key target in neurobiological research, abnormal processing of amyloid precursor protein (APP) is closely related to the pathological progression of Alzheimer's disease (AD). Our in vitro APP processing assay service uses world-leading multidimensional analysis technology to provide accurate data support for drug development and mechanism research. For further information regarding the products and services provided, project-specific consultation, and pricing, please submit an inquiry here.

Introduction

Available Assays at Creative Biolabs

• Dual Route Dynamic Monitoring

Simultaneous detection of the α-secretase non-amyloid pathway (generating neuroprotective sAPPα and p3 peptides) and the β-secretase amyloid pathway (generating pathogenic Aβ). It can identify key protease activities such as ADAM10, BACE1 and γ-secretase. Detection sensitivity reaches the pg level.

Fig.1 In iPSC-derived cortical neurons, secreted SAPP and Aβ were quantified by immunochemistry.²

• Multi-Dimensional Detection Platform

We offer a variety of detection methods, including but not limited to Chemiluminescent Immunoassay (CLIA): specificity > 90%, supports multiple sample types such as serum/plasma/cerebrospinal fluid. Enzyme-linked immunosorbent assay (ELISA) technology: can distinguish between full-length APP protein and its cleavage fragments (C83/C99). Mass spectrometry verification service: accurate identification of Aβ subtypes (such as Aβ40/Aβ42).

• Detailed Analysis of Pathological Mechanisms

Our scientists also provide analytical services, including but not limited to: detecting the effect of ubiquitination modification on APP metabolism (regulated by ligases such as VHL/HRD1), analyzing the damage of β/γ secretase co-localization on cell function, and evaluating the non-toxic cleavage of APP by metalloproteinases (such as Meprin β).

Featured Services

• We offer various subtypes of recombinant proteins such as APP770 and APP695 (purity > 95%).
• Support for antibody validation services: coverage of N-terminal/C-terminal epitopes, support for the entire process including WB/IF/IP.
• Personalized solution design: optimize detection conditions for different models such as neurons/cell lines/tumor cells.

Our company has 10 years of experience in neurobiological testing, and our service network covers more than 30 research institutions around the world. All experiments provide original data and bioinformatics analysis reports. Please contact us immediately to obtain customized testing solutions and technical white papers to help you break through the bottleneck of AD research!

References

1. Zhang, Yun-wu, et al. "APP processing in Alzheimer's disease." Molecular brain 4 (2011): 1-13. Distributed under Open Access license CC BY 2.0, without modification.
2. Bergström, Petra, et al. "Amyloid precursor protein expression and processing are differentially regulated during cortical neuron differentiation." Scientific reports 6.1 (2016): 29200. Distributed under Open Access license CC BY 4.0, without modification.