C. elegans Transgenic Service
Creative Biolabs offers state-of-the-art C. elegans transgenic services to enable researchers to explore the vast potential of this remarkable organism. Our C. elegans Transgenic Service empowers scientists to explore and unravel the intricate mechanisms underlying various biological phenomena. With our expertise and advanced techniques, we aim to accelerate discoveries and drive scientific progress in the field.
CRISPR/Cas9 Gene Editing Service in C. elegans
The revolutionary CRISPR/Cas9 system has transformed the field of genetic engineering by providing a precise and efficient method for modifying genomes. At Creative Biolabs, we have harnessed the power of CRISPR/Cas9 to offer a state-of-the-art C. elegans gene editing service. Using CRISPR/Cas9, we can precisely target and modify specific genes of interest in the C. elegans genome. This technology allows us to introduce or delete genetic sequences, knock down gene expression, or create precise mutations. Specifically, we can provide the following gene editing using the CRISPR/Cas9 system:
- Precise sequence/gene deletion (≤1500bp)
- Precise nucleotide changes
- Precise sequence knock-in (≤900bp)
- Precise sequence replacement
- 2xLoxP knock-in
- 2xFRT knock-in
Table 1. Procedures of CRISPR/Cas9 gene editing services.
| Procedures | Materials | Standard |
| Reagent preparation | sgRNA plasmids, Cas9 plasmid and repair templates | High quality plasmids and templates |
| Microinjection | The designated C. elegans strains | 10-30 adults |
| Screening | F1 and/or F2 progeny of injected worms | Animals with the desired editing |
| Final verification | Animals containing the desired sequence changes | Verification by sequencing |
Traditional C. elegans Transgenic Services
Traditional transgenic services encompass the production of extrachromosomal arrays and integrated arrays. Through these services, transgenic animals with multiple copies of transgenes can be generated, leading to an increased expression of the desired plasmid.
The process of these services involves initiating microinjection of a DNA mixture into the gonads of C. elegans. This step aims to create extrachromosomal transgenic arrays that contain numerous copies of the injected DNA constructs. Additionally, for X-ray mediated integrated arrays, the extrachromosomal arrays undergo X-ray irradiation and subsequent screening to identify integrated arrays. The integration site occurs randomly and can be determined by employing mapping techniques.
Fig.1 Extrachromosomal arrays for mosaic analysis in C. elegans. (Yochem, 2003)
Vector modifications, such as replacing promoters, coding sequences, or 3' UTR sequences, as well as sub-cloning and site-directed mutagenesis, are frequently employed techniques in molecular biology experiments. Creative Biolabs specializes in delivering effective services for vector modification, sub-cloning, and site-directed mutagenesis, tailored to meet the specific requirements of our customers. Our expertise extends to performing vector modifications using a ligation-independent approach, ensuring that no extra restriction enzyme sites or linker sequences are introduced during the process.
Table 2. Traditional C. elegans Transgenic Services.
| Service | You need to provide | Deliverables |
| Plasmid Construction |
1. Information of the vector backbone 2. The modification you need |
Plasmid and sequencing reports |
| Extra-chromosomal arrays |
1. Target plasmid and it's concentration 2. The marker and it's concentration 3. Background strain information |
Extrachromosomal lines that contain the target plasmid and report markers |
| X-ray mediated integrated arrays | Integrated lines that contain the target plasmids and report markers |
Our experienced team of scientists possesses profound expertise in C. elegans transgenesis and has successfully generated numerous transgenic lines for various research purposes. Please feel free to contact us for more about our C. elegans models related services.
References
- Kim, Hyun-Min & Colaiácovo, Monica. (2016). CRISPR-Cas9-Guided Genome Engineering in C. elegans: CRISPR-Cas9 Genome Engineering in C. elegans. 10.1002/cpmb.7.
- Yochem, John and Robert K. Herman. "Investigating C. elegans development through mosaic analysis." Development (2003).
