Zebrafish Transgenic Modeling Service
Zebrafish (Danio rerio) is s a powerful model organism for studying various aspects of neuroscience due to its unique attributes, including its rapid development, transparency during early stages, and genetic similarity to humans. Zebrafish transgenic technology, which involves introducing foreign genes into their genome, has revolutionized the study of neurobiology. Creative Biolabs offers a comprehensive list of Zebrafish Transgenic Models, empowering researchers with advanced tools to unravel the mysteries of the nervous system.
Zebrafish Gene Manipulation Platform
Creative Biolabs' expert team employs state-of-the-art gene manipulation techniques to introduce foreign genes or modify endogenous genes in zebrafish embryos. These techniques include:
- Pronuclear Microinjection: This classic method involves injecting DNA constructs directly into the pronuclei of fertilized zebrafish eggs. By carefully controlling the timing and concentration of the injected DNA, our scientists can achieve efficient integration of the foreign genes into the zebrafish genome.
- CRISPR/Cas9-Mediated Genome Editing: The revolutionary CRISPR/Cas9 system has transformed the field of genetic engineering. By utilizing this powerful tool, our experts can precisely edit zebrafish genomes, enabling the creation of precise gene knockouts, knock-ins, or other modifications. This technique allows researchers to investigate the functions of specific genes and study their role in neurodevelopment, behavior, and disease.
- Transposon-Mediated Transgenesis: Transposons, such as Tol2 and Sleeping Beauty, are mobile genetic elements capable of integrating foreign DNA into the zebrafish genome. Creative Biolabs utilizes transposon-mediated transgenesis to achieve stable integration of transgenes, resulting in heritable genetic modifications in zebrafish offspring.
Zebrafish Gene Expression and Visualization
Creative Biolabs provides researchers with various techniques to visualize gene expression in zebrafish, enabling the study of gene activity patterns in the developing brain. Notable methods utilized by Creative Biolabs include:
- Fluorescent Reporter Systems: By incorporating fluorescent reporter genes, such as green fluorescent protein (GFP), into the zebrafish genome, we can visualize and track the expression of specific genes in real-time. This technique enables researchers to precisely identify neural cell types, monitor cell migration, and study neuronal connectivity during development.
- Cell-Specific Promoter Analysis: Our experts can generate zebrafish lines that express reporter genes under the control of cell-specific promoters. This approach facilitates the identification and characterization of specific cell populations within the nervous system, enabling targeted studies on neuronal subtypes or glial cells.
- In Situ Hybridization: In situ hybridization is a powerful technique used to visualize the spatial distribution of RNA transcripts in zebrafish tissues. By utilizing complementary DNA probes labeled with specific markers, researchers can identify the regions where specific genes are active in the developing nervous system.
Fig.1 Synaptogenesis imaging of transgenic zebrafish model. (Du, 2018)
Our Transgenic Zebrafish Models with Neurosystem-Specific Markers
Creative Biolabs provides researchers with an extensive range of transgenic zebrafish models expressing specific markers for the neurosystem, aiding in the advancement of neuroscience research. These models offer a valuable opportunity to investigate and understand the intricate workings of the nervous system. Here are some notable models worth mentioning:
Cat.# | Genotype | Construct | Note |
NRZP-0523-ZP1 | ihb27Tg/+ | Tg(OTM:GFP) | Optimal Tcf motif, TOP driving GFP expression, midbrain GFP |
NRZP-0523-ZP2 | zf44Tg/+ | Tg(-1.0pomca:GFP) | pituitary |
NRZP-0523-ZP3 | ihb5Tg/+ | Tg(-2.5tshb:EGFP) | pituitary/pineal/thyroid |
NRZP-0523-ZP4 | Tg(atoh7:Gal4); Tg(UAS:mcherry) | Tg(atoh7:Gal4); Tg(UAS:mcherry) | retinal ganglion cells |
NRZP-0523-ZP5 | cf2Tg/+ | Tg(elavl3:YC2) | neurons, retina |
NRZP-0523-ZP6 | ml3Tg/+ | Tg(mnx1:mGFP) | Primary motor axons, motoneurons |
NRZP-0523-ZP7 | rw0Tg/+ | Tg(isl1:GFP) | cranial motor neuron |
NRZP-0523-ZP8 | zf147Tg/+ | Tg(apoeb:LY-EGFP) | Neuronal |
NRZP-0523-ZP9 | kca66Tg/+ | Tg(h2afva:h2afva-GFP) | neuromast primordium migration, posterior lateral line nerve development |
NRZP-0523-ZP10 | ck2Tg/+ | Tg(eef1a1l1:Kaede) | Brain, hatching gland |
NRZP-0523-ZP11 | jt0012Tg/+ | Tg(ompb:tau-EGFP) | Olfactory bulb, Olfactory placode |
NRZP-0523-ZP12 | ck1Tg/+ | Tg(mbp:EGFP) | myelinating glia, central nervous system |
NRZP-0523-ZP13 | vu19Tg/+ | Tg(olig2:DsRed2) | Oligodendrocyte, motor neuron |
NRZP-0523-ZP14 | Tg(mbp:mGFP) | Tg(mbp:mGFP) | myelinating glia |
NRZP-0523-ZP15 | knu3Tg/+ | Tg(elavl3:EGFP) | neurons, nervous system |
NRZP-0523-ZP16 | Tg(ift46:GAL4-VP16;UAS:nfsB-mCherry) | Tg(ift46:GAL4-VP16;UAS:nfsB-mCherry) | eye, pronephric duct, spinal cord |
NRZP-0523-ZP17 | nju1Tg/+ | Tg(cyp26a1:EYFP) | retina,olfactory vesicle, anterior dorsal spinal cord,proctodeum,caudal notochord,pharyngeal arches |
NRZP-0523-ZP18 | nns8Tg/+ | Tg(atoh1a:dTomato) | cerebellum |
NRZP-0523-ZP19 | nl1Tg/+ | TgBAC(neurod1:EGFP) | Cranial ganglia, lateral line nerve cells |
NRZP-0523-ZP20 | um14Tg/+ | Tg(EPV.Tp1-Mmu.Hbb:EGFP) | Notch-responsive tissues such as the developing CNS, vasculature, liver, intestine and pancreas |
NRZP-0523-ZP21 | Tg(huc:YFP)/+ | Tg1(elavl3:YFP) | neurons |
NRZP-0523-ZP22 | Tg(crybb1:CFP)/+ | Tg(crybb1:CFP) | eye lens |
NRZP-0523-ZP23 | ihb304Tg/+ | Tg(gad1b:mCherry) | olfactory pit, optic tectum, medulla oblong, eye, spinal cord |
Please feel free to contact us for more about our transgenic zebrafish models.
Reference
- Du, Xu-fei, et al. "A transgenic zebrafish model for in vivo long-term imaging of retinotectal synaptogenesis." Scientific Reports 8.1 (2018): 14077.
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