Tau Hyperphosphorylation Assay Service

We provide you with the world's leading tau hyperphosphorylation assay service based on multiple technologies to support Alzheimer's disease (AD) drug development, disease monitoring, and mechanism research. For further information regarding the products and services provided, project-specific consultation, and pricing, please submit an inquiry here.

Hyperphosphorylated tau and AD

Hyperphosphorylation of tau protein is the core pathological mechanism of neurodegenerative diseases such as AD. Normal tau protein maintains neuronal function by regulating microtubule homeostasis, while abnormal phosphorylation causes it to dissociate from microtubules and form neurofibrillary tangles (NFTs), triggering neuronal apoptosis and decline in brain function.

Tau hyperphosphorylation has been demonstrated to interfere with ER-Golgi transport, induce ER stress, upregulate GSK3β activity, and further promote Tau phosphorylation. The manifestation of neuronal apoptosis and degeneration is characterized by a decreased survival rate and a significant presence of degenerating neurons rich in NFTs.

Fig.1 Tau hyperphosphorylation.^{1, 3}

Multiple phosphorylation site-sensitive detection solution

• Immunological Methods

We offer enzyme-linked immunosorbent assay (ELISA), immunohistochemistry (IHC), and other technologies to quantitatively analyze several key phosphorylation sites of the tau protein, including Thr217, Thr231, Ser396, and Ser404. Abnormal phosphorylation of these sites is strongly associated with the progression of AD.

• Technical Characteristics

Our service is highly specific and utilizes phosphorylated tau antibodies developed by Creative Biolabs and covers samples from multiple species (human, mouse, rat, etc.). Combined detection of amyloid beta (Aβ) and tau protein significantly improves AD specificity.

• Cell-based Model Analysis

Based on our powerful in vitro platform, we also develop cell models (such as tau-overexpressing HEK293 cells) to study phosphorylation dynamics and pathological effects. For example, hyperphosphorylated tau can resist apoptosis but promote chronic neurodegeneration.

Fig.2 AD P-Tau-induced site-specific hyperphosphorylation of tau in the hippocampus of mice.^{2, 3}

Service Features

• Global Supply Chain: As a global CRO company, we look for the best test kits. We provide you with stable technical support and testing services.
• Full Process Service: Meeting the full needs of the scientific research process, from sample collection and transport to data interpretation.
• Sample Compatibility: Applicable to various sample types such as cerebrospinal fluid, serum, cell culture supernatant, etc. to meet different research needs.

Tau hyperphosphorylation assay is a critical tool for the study of neurodegenerative diseases. It provides in-depth insights into the phosphorylation mechanism of tau protein, thereby facilitating breakthroughs in the diagnosis and treatment of neurodegenerative diseases. We are committed to providing our customers with efficient and accurate testing services to support scientific research, innovation, and disease treatment. We look forward to your inquiries.

References

1. Rawat, Priyanka, et al. "Phosphorylated tau in Alzheimer’s disease and other tauopathies." International Journal of Molecular Sciences 23.21 (2022): 12841.
2. Miao, Jin, et al. "Pathological tau from Alzheimer’s brain induces site-specific hyperphosphorylation and SDS-and reducing agent-resistant aggregation of tau in vivo." Frontiers in aging neuroscience 11 (2019): 34.
3. Distributed under Open Access license CC BY 4.0, without modification.