NeuroMab™ Anti-OPN BBB Shuttle Antibody(NRZP-1022-ZP2608)

Fig.1 shows the determination of ECso for the four clones in the peptide antigen binding assay.

The data are shown as mean±SE for 8 animals, 7 animals and 5 animals in the control group, 25 mg/kg group and 50 mg/kg group, respectively. *p<0.05, **p<0.01: significantly different from control group as determined by Dunnet's multiple comparison test.

Fig.2 shows the EC50 values for the four clones for binding to the OPN antigen peptide.

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.

Fig.3 shows the % inhibition for the four clones in inhibiting the cleavage of human OPN-FL

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.

Fig.4 shows the determination of inhibition of thrombin cleavage of mouse OPN-FL for the four clones by determining generation of one of the cleavage products, OPN-R, in a specific ELISA for OPN-R.

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.

Fig.5 shows the determination of EC50 values for the four clones in the direct binding ELISA against mouse OPN-FL.

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.