NeuroMab™ Anti-OPN BBB Shuttle Antibody(NRZP-1022-ZP2611)

Fig.1 shows the determination of ECso for the four clones in the peptide antigen binding assay.

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.

Fig.2 shows the EC50 values for the four clones for binding to the OPN antigen peptide.

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.

Fig.3 shows the % inhibition for the four clones in inhibiting the cleavage of human OPN-FL

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.

Fig.4 shows the determination of inhibition of thrombin cleavage of mouse OPN-FL for the four clones by determining generation of one of the cleavage products, OPN-R, in a specific ELISA for OPN-R.

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.

Fig.5 shows the determination of EC50 values for the four clones in the direct binding ELISA against mouse OPN-FL.

EC50 values were calculated from the 4-parameter fit equation using Prism Graphpad using the data from FIG.10. A2=2.6 ng/mL, A6=3.2 ng/mL; E5=4.0 ng/mL and G7=93.2 ng/mL.