Alkaline Phosphatase Staining Kit
[CAT#: NCC200551ZP]
A specific and sensitive tool for phenotypic assessment of ES/iPS cell differentiation by determining AP activity.
- Applications:
- Stem Cell Culture
- Cell Types:
- Embryonic Stem Cell; iPSC
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
The undifferentiated state of embryonic stem cells (ES) and induced pluripotent stem cells (iPS) can be characterized by high-level expression of alkaline phosphatase (AP), which together with the expression of other surface markers indicates that undifferentiated cells have The potential for self-differentiation-renewal. AP is a hydrolase enzyme that is responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When an alkaline phosphatase staining kit is used to stain fixed ES or iPS cells, undifferentiated cells appear blue and differentiated cells appear colorless.
Species Reactivity
Human; Mouse; Rat
Cell Types
Embryonic Stem Cell; iPSC
Applications
Stem Cell Culture
Application Notes
Other materials required:
Fixative (eg 4% paraformaldehyde in PBS)
1X PBS
Fixative (eg 4% paraformaldehyde in PBS)
1X PBS
Research Areas
Stem Cell Research
Properites
Components
AP Staining Solution A
AP Staining Solution B
AP Staining Solution B
Preparation and Storage
Shipping
Dry Ice
Storage
4°C
Shelf Life
This product is stable for 12 months when stored as directed.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Publications
Publications (0)
For Research Use Only. Not For Clinical Use.
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