Alkaline Phosphatase Staining Kit

[CAT#: NCC200551ZP]

A specific and sensitive tool for phenotypic assessment of ES/iPS cell differentiation by determining AP activity.

Applications:: Stem Cell Culture

Cell Types:: Embryonic Stem Cell; iPSC

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Description

The undifferentiated state of embryonic stem cells (ES) and induced pluripotent stem cells (iPS) can be characterized by high-level expression of alkaline phosphatase (AP), which together with the expression of other surface markers indicates that undifferentiated cells have The potential for self-differentiation-renewal. AP is a hydrolase enzyme that is responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When an alkaline phosphatase staining kit is used to stain fixed ES or iPS cells, undifferentiated cells appear blue and differentiated cells appear colorless.