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Creative Biolabs

Alkaline Phosphatase Staining Kit

[CAT#: NCC200551ZP]

A specific and sensitive tool for phenotypic assessment of ES/iPS cell differentiation by determining AP activity.

Applications:
Stem Cell Culture
Cell Types:
Embryonic Stem Cell; iPSC

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Product Overview

Description

The undifferentiated state of embryonic stem cells (ES) and induced pluripotent stem cells (iPS) can be characterized by high-level expression of alkaline phosphatase (AP), which together with the expression of other surface markers indicates that undifferentiated cells have The potential for self-differentiation-renewal. AP is a hydrolase enzyme that is responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. When an alkaline phosphatase staining kit is used to stain fixed ES or iPS cells, undifferentiated cells appear blue and differentiated cells appear colorless.

Species Reactivity

Human; Mouse; Rat

Cell Types

Embryonic Stem Cell; iPSC

Applications

Stem Cell Culture

Application Notes

Other materials required:
Fixative (eg 4% paraformaldehyde in PBS)
1X PBS

Research Areas

Stem Cell Research
Properites

Components

AP Staining Solution A
AP Staining Solution B
Preparation and Storage

Shipping

Dry Ice

Storage

4°C

Shelf Life

This product is stable for 12 months when stored as directed.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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