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- Mouse Anti-SCN5A Monoclonal Antibody (CBP708) (Cat#: NAB-0720-Z2720)
- NeuroMab™ Anti-TNFα BBB Shuttle Antibody,Clone NR3088P (Cat#: NRZP-1022-ZP4105)
- NeuroMab™ Anti-Alpha Synuclein Antibody,Clone NR3861P (Cat#: NRP-0422-P614)
- NeuroMab™ Anti-Alpha Synuclein BBB Shuttle Antibody,Clone NR1707P (Cat#: NRZP-1022-ZP4050)
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- NeuroMab™ Anti-ApoC3 BBB Shuttle Antibody,Clone NR1616P (Cat#: NRZP-1022-ZP3505)
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- Rat Microglia Cell Line HAPI, Immortalized (Cat#: NCL2110P015)
- Human Microglia Cell Line HMC3, Immortalized (Cat#: NCL-2108P38)
- Sf295 Human Glioblastoma Cells (Cat#: NCL-2108P180)
- iNeu™ Human Schwann Cell (Cat#: NCL-2103-P63)
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- Beta Amyloid (1-40), Aggregation Kit, TTF Assay (Cat#: NRZP-0323-ZP199)
- Human Tau Aggregation Kit (Cat#: NRP-0322-P2173)
- Beta Amyloid (1-42), Aggregation Kit (Cat#: NRZP-0323-ZP200)
- Alpha Synuclein Aggregation Kit (Cat#: NRZP-1122-ZP15)
- Alpha-Synuclein Aggregation Assay Kit (Cat#: NRZP-1122-ZP37)
- Human Poly ADP ribose polymerase,PARP Assay Kit (Cat#: NRZP-1122-ZP62)
- Human GFAP ELISA Kit [Colorimetric] (Cat#: NPP2011ZP383)
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FZStem™ Stem Cell Freezing Medium
Used for the cryopreservation of human embryonic stem (hES) cells and induced pluripotent stem (iPS) cells.
- Applications:
- Cell Cryopreservation
- Cell Types:
- Embryonic Stem Cell; iPSC
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Handling Advices
2. Culture the cells in a 6-well plate until 60% to 80% confluent.
3. Aspirate the medium from the hES/hiPS cell culture and rinse with DPBS (2 mL/well).
4. Add 0.5 mL FZStem enzyme-free stem cell dissociation solution to each well. Let it stand at room temperature for 1-2 minutes.
5. Aspirate the dissociation solution, and then gently rinse each well with 2 mL DMEM/F-12 2-3 times.
6. Add 2 mL/well fresh medium, and scrape the colonies with a cell scraper.
7. Transfer the separated cell suspension to a 15 mL conical tube.
8. Centrifuge at 200 x g for 5 minutes at room temperature.
9. Aspirate the supernatant gently and loosen the cell pellet by tapping on the bottom of the test tube.
10. Gently resuspend the pellet in cold FZStem freezing medium, taking care to make the clumps larger than the usual passaging process.
11. Transfer 1 mL of cell suspension to each labeled cryogenic vial.
12. Place the vial in an isopropanol freezing container, and place the container at -80°C overnight.
13. Transfer to the liquid nitrogen tank the next day.
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