iNeuMab™ Anti-Alpha Synuclein Antibody (NRP-0422-P623)

Q: What is the recommended dilution for using the antibody in Western Blotting?

For Western Blotting, we recommend using the antibody at a dilution of 1:1,000 to 1:5,000. However, optimal dilutions may vary depending on the specific experimental conditions and sample types. It’s advisable to perform initial titration experiments to determine the best dilution for your particular setup to achieve optimal signal-to-noise ratio.

Q: Are there any troubleshooting tips for using the antibody in IP experiments?

While the antibody is effective in IP applications, it’s crucial to use optimal conditions to achieve the best results. Ensure that the antibody-to-protein ratio is appropriate and that the lysis buffer used does not interfere with antibody binding. Additionally, the use of proper washing steps is important to reduce non-specific binding. If issues arise, troubleshooting might involve optimizing these parameters or adjusting incubation times.

Q: How should I prepare my samples for optimal results using the antibody in Western Blotting?

For optimal results in Western Blotting, prepare your samples by lysing cells or tissues in an appropriate lysis buffer, such as RIPA buffer, supplemented with protease inhibitors. Ensure that the protein concentration is accurately measured, and load equal amounts of protein per lane. Denature the samples by boiling in SDS-PAGE sample buffer with reducing agents, then resolve the proteins by SDS-PAGE, transfer to a PVDF or nitrocellulose membrane, and follow with antibody incubation.

Product Overview

Description

NRP-0422-P623 (monoclonal) specific for the C-terminus of SN1-119.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Applications

WB; IHC; IP; ELISA

Relevant Diseases

Parkinson's Disease

Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only

Target

AlphaSynuclein

Official Name

SNCA

Full Name

Alpha-synuclein

Alternative Names

SNCA; NACP; PARK1; PARK4; PD1; synuclein alpha

Gene ID

6622 (Human); 20617 (Mouse)

Uniprot ID

P37840

Publications

Publications (0)

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Customer Reviews and Q&As

Customer Reviews Average Customer Ratings Overall

5.0

Excellent

Highly recommend it for anyone working on alpha-synuclein

The antibody's specificity is remarkable, giving us clean bands and clear staining patterns. It has significantly reduced background noise in our experiments, making data interpretation much easier.

Excellent

A game-changer for our lab

We will definitely continue using this antibody in our studies. The results are consistently reproducible, and the antibody's affinity for alpha-synuclein is impressive. The quality of this product has made a noticeable difference in the accuracy of our research findings.

Excellent

Top-notch

It consistently delivers strong, specific signals with minimal background. The antibody's performance in IP has also been stellar, helping us isolate high-quality protein complexes.

Excellent

It consistently produces high-quality results across WB, IHC, and IP applications

The antibody's ability to detect different forms of alpha-synuclein has been particularly useful for our research. The clear, strong signals have greatly facilitated our studies on protein aggregation.

Excellent

A fantastic tool

It has proven to be extremely reliable in WB and IHC, delivering consistent and reproducible results. The antibody's high specificity has allowed us to differentiate between various isoforms of alpha-synuclein, which is crucial for our research. This is definitely a product we trust and recommend.

Q&As

What is the recommended dilution for using the antibody in Western Blotting?

For Western Blotting, we recommend using the antibody at a dilution of 1:1,000 to 1:5,000. However, optimal dilutions may vary depending on the specific experimental conditions and sample types. It’s advisable to perform initial titration experiments to determine the best dilution for your particular setup to achieve optimal signal-to-noise ratio.

Are there any troubleshooting tips for using the antibody in IP experiments?

While the antibody is effective in IP applications, it’s crucial to use optimal conditions to achieve the best results. Ensure that the antibody-to-protein ratio is appropriate and that the lysis buffer used does not interfere with antibody binding. Additionally, the use of proper washing steps is important to reduce non-specific binding. If issues arise, troubleshooting might involve optimizing these parameters or adjusting incubation times.

How should I prepare my samples for optimal results using the antibody in Western Blotting?

For optimal results in Western Blotting, prepare your samples by lysing cells or tissues in an appropriate lysis buffer, such as RIPA buffer, supplemented with protease inhibitors. Ensure that the protein concentration is accurately measured, and load equal amounts of protein per lane. Denature the samples by boiling in SDS-PAGE sample buffer with reducing agents, then resolve the proteins by SDS-PAGE, transfer to a PVDF or nitrocellulose membrane, and follow with antibody incubation.