NeuroMab™ Mouse Anti-CD86 Monoclonal Antibody (CBP2151)

Product Overview

Description

CD86, known as B7-2, B70, and Ly-58, is a member of immunoglobulin superfamily. It is expressed on the surface of B lymphocytes, T lymphocytes, dendritic cells and astrocytes. As a ligand for CD28 and CD152 (CTLA-4), CD86 plays a critical role in T cell-B cell interactions.
The interaction of CD86 with CD28 can transduce co-stimulatory signals for T cell proliferation and cytokine production. CD86 is also involved in NK cell-mediated cytotoxicity.

Species Reactivity

Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

Mouse

Clone Number

CBP2151

Applications

ELISA

Application Notes

Working concentrations for specific applications should be determined by the investigators. The appropriate concentration may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined.
The following concentration ranges are recommended starting points for this product.

ELISA detection: 0.01-0.05 µg/ml

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1611

Product Properties

Concentration

0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide.

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

The lyophilized product remains stable up to 1 year at -20 °C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid freeze/thaw cycles.

Handling Advice

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

CD86

Official Name

CD86

Full Name

CD86 molecule

Alternative Names

CD86; CD86 molecule; B70; B7-2; B7.2; LAB72; CD28LG2; T-lymphocyte activation antigen CD86; BU63; FUN-1; CTLA-4 counter-receptor B7.2; B-lymphocyte activation antigen B7-2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)

Data