NeuroMab™ Mouse Anti-POU5F1 Monoclonal Antibody (CBP2650)

Product Overview

Description

Octamer-binding protein 4 (OCT4) is a member of the POU gene family and encoded by the human gene POU5F1 (POU domain class 5 transcription factor 1). OCT4 is a member of the Octamer class of transcription factors that recognize the 8-base pair consensus motif 5'-ATGCAAAT-3'. Expression of OCT4 is associated with an undifferentiated, pluripotent stem cell phenotype and tumors. OCT4 is also expressed in the developing brain, with the highest levels found in the cortex, olfactory bulb, and the hippocampus.

Expression Host

Mammalian cells

Immunogen

OCT4 fusion protein Ag1794

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2650

Applications

IHC; WB; ELISA

Application Notes

IHC : 1:20-1:200; WB : 1:500-1:1000

Research Areas

Neuron

Conjugation

Unconjugated; APC; PE; HRP; Biotin; FITC; Alexa Fluor 488; Alexa Fluor 700; Alexa Fluor 647; Alexa Fluor 750; Alexa Fluor 594; Alexa Fluor 350; Alexa Fluor 1887

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1000 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Endotoxin Level

Low Endotoxin

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

POU5F1

Official Name

POU5F1

Full Name

POU class 5 homeobox 1

Alternative Names

Oct 3; Oct 4; OCT3; OCT3/4; OCT4; Octamer binding protein 3; Octamer binding protein 4; OTF 3; OTF3

Gene ID

5462(Human)

Uniprot ID

Q06416(Human)

References

1. Xie, C., Zhu, J., Wang, X., Chen, J., Geng, S., Wu, J.,... & Li, X. (2019). Tobacco smoke induced hepatic cancer stem cell-like properties through IL-33/p38 pathway. Journal of Experimental & Clinical Cancer Research, 38(1), 1-13.

Publications