Cell Immortalization Kit, hTERT Overexpressed (Human)

[CAT#: NCC200553ZP]

Applications:: Cell Immortalization

Cell Types:: Neural Lineage Cell

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Product Overview

Description

It is well known that primary cells undergo only a predetermined and limited number of cell divisions in culture. After doubling the population (the number varies with species, cell type, and culture conditions), the primary cells enter a so-called state of replication senescence, in which they no longer divide. To overcome aging, there are several methods for immortalizing mammalian cells in culture. The latest discovery of cell immortalization is through the expression of telomerase reverse transcriptase protein (TERT). It is particularly useful for cells that are most affected by telomere length, including many human cell types. This protein is usually silent in most somatic cells. When hTERT is introduced exogenously, these cells can avoid replication senescence by maintaining sufficient telomere length. However, overexpression of hTERT in certain cell types (especially in epithelial cells) cannot induce cell immortalization.

Cell Types

Neural Lineage Cell

Species Reactivity

Human

Applications

Cell Immortalization

Research Areas

Stem Cell Research

Properties

Shipping

Dry Ice

Storage

Store at -80 °C

Quality Control

The packaged lentivector construct is provided as frozen VSV-G pseudotype virus particles. The titer of the lentivirus was measured by qRT-PCR titration kit. Each batch of cell immortalization kits were functionally tested in fibroblasts.

Shelf Life

Stable up to 6 months

Research Use Only

For research use only, not for diagnostic or therapeutic use.