Creative Biolabs

Cell Immortalization Kit, hTERT Overexpressed (Human)

[CAT#: NCC200553ZP]

Applications:
Cell Immortalization
Cell Types:
Neural Lineage Cell

Datasheet MSDS Request COA

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Bulk Quote
Description
It is well known that primary cells undergo only a predetermined and limited number of cell divisions in culture. After doubling the population (the number varies with species, cell type, and culture conditions), the primary cells enter a so-called state of replication senescence, in which they no longer divide. To overcome aging, there are several methods for immortalizing mammalian cells in culture. The latest discovery of cell immortalization is through the expression of telomerase reverse transcriptase protein (TERT). It is particularly useful for cells that are most affected by telomere length, including many human cell types. This protein is usually silent in most somatic cells. When hTERT is introduced exogenously, these cells can avoid replication senescence by maintaining sufficient telomere length. However, overexpression of hTERT in certain cell types (especially in epithelial cells) cannot induce cell immortalization.
Cell Types
Neural Lineage Cell
Species Reactivity
Human
Applications
Cell Immortalization
Research Areas
Stem Cell Research
Shipping
Dry Ice
Storage
Store at -80 °C
Quality Control
The packaged slow vector construct is provided as frozen VSV-G pseudotype virus particles. The titer of the lentivirus was measured by qRT-PCR titration kit. Each batch of cell immortalization kits were functionally tested in fibroblasts.
Shelf Life
Stable up to 6 months
Research Use Only
For research use only, not for diagnostic or therapeutic use.

For Research Use Only. Not For Clinical Use.
Send Inquiry Send Inquiry
Inquiry Basket
compare

Go to compare