Creative Biolabs

Human CAST Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP68]

Human CAST knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HeLa

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Description
This is a HeLa cell transfected lysate in which Human CAST has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HeLa
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 12 and Insertion of the selection cassette in exon 12.
Passage Number
<20
Knockout Validation
Sanger Sequencing
Relevant Diseases
Neurological Disorders
Research Areas
Neurotransmission
Pathways
Secretory Signaling
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8, 12
CSF1PO: 9, 10
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
Calpastatin
Official Name
CAST
Full Name
Calpastatin
Alternative Names
CAST; BS-17; PLACK; calpastatin
Gene ID
831 (Human); 12380 (Mouse)
Uniprot ID
P20810 (Human); P51125 (Mouse)
For Research Use Only. Not For Clinical Use.
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