Mouse Anti-HSPA1A Monoclonal Antibody (CBP2708)

Product Overview

Description

HSPA1A is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker.

Expression Host

Mammalian cells

Immunogen

HSP70 fusion protein Ag19111

Species Reactivity

Human; Yeast; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2708

Applications

IF; IHC; WB; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; WB : 1:500-1:1000

Research Areas

Neural Signal Transduction

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

880 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

HSPA1A

Official Name

HSPA1A

Full Name

Heat shock 70kDa protein 1A

Alternative Names

Heat shock 70 kDa protein 1/2; heat shock 70kDa protein 1A; HSP70; HSP70 1; HSP70 1/HSP70 2; HSP70 1A

Gene ID

3303(Human); 3304(Mouse); 24472(Rat)

Uniprot ID

P0DMV9(Human); P17879(Mouse); P0DMW1(Rat)

References

1. Xiaohua, D., Weili, W., Xiaoqing, L., Hanwen, Y., Rong, D., & Qing, L. (2015). Neuroprotective effect of ethyl acetate extract from gastrodia elata against transient focal cerebral ischemia in rats induced by middle cerebral artery occlusion. Journal of Traditional Chinese Medicine, 35(6), 671-678. 2. Li, L., Yan, S., Zhang, H., Zhang, M., Huang, G., & Chen, M. (2019). Interaction of hnRNP K with MAP 1B-LC1 promotes TGF-β1-mediated epithelial to mesenchymal transition in lung cancer cells. BMC cancer, 19(1), 894. 3. Singla, S., Iwamoto-Stohl, L. K., Zhu, M., & Zernicka-Goetz, M. (2020). Autophagy-mediated apoptosis eliminates aneuploid cells in a mouse model of chromosome mosaicism. Nature Communications, 11(1), 1-15.

Publications