Mouse Anti-TBP Monoclonal Antibody (2H3B2), Unconjugated

[CAT#: NAB20101780CR]

Mouse Monoclonal (2H3B2) to TBP

Host Species:: Mouse

Species Reactivity:: Human; Mouse; Rat; Porcine

Applications:: FC; IF; IHC; IP; WB; ELISA

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Product Overview

Description

The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This antibody recognize human TBP with MW 40 kDa and mouse/rat Tbp with MW 34 kDa.

Expression Host

Mammalian cells

Immunogen

TBP fusion protein Ag12383

Species Reactivity

Human; Mouse; Rat; Porcine

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

2H3B2

Applications

FC; IF; IHC; IP; WB; ELISA

Application Notes

IF : 1:20-1:200; IHC : 1:20-1:200; IP : 0.5-4.0 ug; WB : 1:500-1:1000

Relevant Diseases

Neurological Disorders

Research Areas

Neurogenesis

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1500 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

TBP

Official Name

TBP

Full Name

TATA box binding protein

Alternative Names

GTF2D;
GTF2D1;
HDL4;
MGC117320;
MGC126054;
MGC126055;
SCA17;
TATA binding factor;
TATA box factor;

Gene ID

6908(Human); 21374(Mouse); 117526(Rat)

Uniprot ID

P20226(Human); P29037(Mouse)

References

1. Zhang, M., Deng, X., Guan, X., Geng, L., Fu, M., Zhang, B.,... & Li, M. (2018). Herpes simplex virus type 2 infection-induced expression of CXCR3 ligands promotes CD4+ T cell migration and is regulated by the viral immediate-early protein ICP4. Frontiers in immunology, 9, 2932. 2. Lyu, C., Zhang, Y., Gu, M., Huang, Y., Liu, G., Wang, C.,... & Gu, Y. (2018). IRAK-M Deficiency exacerbates ischemic neurovascular injuries in experimental stroke mice. Frontiers in cellular neuroscience, 12, 504. 3. Fukumoto, J., Sidramagowda Patil, S., Krishnamurthy, S., Saji, S., John, I., Narala, V. R.,... & Soundararajan, R. (2019). Altered expression of p63 isoforms and expansion of p63-and club cell secretory protein-positive epithelial cells in the lung as novel features of aging. American Journal of Physiology-Cell Physiology, 316(4), C492-C508.