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Creative Biolabs
Product

NeuroMab™ Anti-Alpha Synuclein Antibody,Clone NR3465P

[CAT#: NRP-0422-P621]

A functional antibody raised against Human Alpha Synuclein.

Host Species:
Human
Species Reactivity:
Human
Applications:
WB; ELISA; IHC; In Vitro; In Vivo

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Product Overview

Description

NI-202.21D11 selectively binds human α-synuclein over β-, γ-synuclein and murine α-synuclein in a direct ELISA. NI-202.21D11 binds to the C-terminal acidic domain of α-synuclein.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Human

Clone Number

NR3465P

Applications

WB; ELISA; IHC; In Vitro; In Vivo

Relevant Diseases

Parkinson's Disease
Product Properties

Formulation

PBS only

Preservatives

BSA Free

Concentration

1mg/mL

Purification

Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line

Purity

>95% by SDS PAGE or HPLC

Endotoxin Level

Regular Endotoxin < 5 EU/mg
Low Endotoxin < 1 EU/mg

Shipping

Gel Packs

Storage

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Research Use Only

For research use only
Target

Target

AlphaSynuclein

Official Name

SNCA

Full Name

Alpha-synuclein

Alternative Names

SNCA; NACP; PARK1; PARK4; PD1; synuclein alpha

Gene ID

6622 (Human); 20617 (Mouse)

Uniprot ID

Product Pictures
ELISA

Figure 1 Recombinant human NI-202.21D11 selectively binds human α-synuclein over β-, γ-synuclein, and mouse α-synuclein in a direct ELISA.

Recombinant NI-202.21D11 binds selectively to α-synuclein, while a pan-synuclein antibody binds to all three synucleins, demonstrating equal encapsulation of the recombinant proteins

ELISA

Figure 2 Recombinant human NI-202.21D11 selectively binds human α-synuclein over β-, γ-synuclein, and mouse α-synuclein in a direct ELISA.

Recombinant NI-202.21D11 is selective for human and mouse α-synuclein. On the other hand, NI-202.12F4 binds human and mouse α-synuclein in direct ELISA.

ELISA

Figure 3 Recombinant NI-202.21D11 preferentially binds densely coated α-synuclein.

Recombinant human α-synuclein was coated onto the ELISA plate at the specified concentration, and passed the direct ELISA (□ 20 μg/ml; □ 20 μg/ml;▴ 5 μg/ml; ♦ 1 μg/ml; 0.25 μg/ml;▾0.1 μg/ml). Determine the half-maximal effective concentration (EC50) for each coating concentration, indicating antibody potency.

WB

Figure 4 Epitope mapping reveals the C-terminal binding epitope within human α-synuclein (aa 117-123 of SEQ ID NO: 1) of NI-202.21D11.

Pepscan analysis revealed that NI-202.21D11 overlapped with peptides B08 (aa 109-123 of SEQ ID NO: 1), B09 (aa 113-127 of SEQ ID NO: 1) and B10 (aa of SEQ ID NO: 117-131) Binding to human α-synuclein of 1) shows that the minimal sequence required for NI-202.21D11 binding is PVDPDNE within human α-synuclein (aa 117-123 of SEQ ID NO: 1).

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