NeuroMab™ Anti-Alpha Synuclein Antibody (NRP-0422-P621)
A functional antibody raised against Human Alpha Synuclein.
- Host Species:
- Species Reactivity:
- WB; ELISA; IHC; In Vitro; In Vivo
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Low Endotoxin < 1 EU/mg
Research Use Only
Figure 1 Recombinant human NI-202.21D11 selectively binds human α-synuclein over β-, γ-synuclein, and mouse α-synuclein in a direct ELISA.
Recombinant NI-202.21D11 binds selectively to α-synuclein, while a pan-synuclein antibody binds to all three synucleins, demonstrating equal encapsulation of the recombinant proteins
Figure 2 Recombinant human NI-202.21D11 selectively binds human α-synuclein over β-, γ-synuclein, and mouse α-synuclein in a direct ELISA.
Recombinant NI-202.21D11 is selective for human and mouse α-synuclein. On the other hand, NI-202.12F4 binds human and mouse α-synuclein in direct ELISA.
Figure 3 Recombinant NI-202.21D11 preferentially binds densely coated α-synuclein.
Recombinant human α-synuclein was coated onto the ELISA plate at the specified concentration, and passed the direct ELISA (□ 20 μg/ml; □ 20 μg/ml;▴ 5 μg/ml; ♦ 1 μg/ml; 0.25 μg/ml;▾0.1 μg/ml). Determine the half-maximal effective concentration (EC50) for each coating concentration, indicating antibody potency.
Figure 4 Epitope mapping reveals the C-terminal binding epitope within human α-synuclein (aa 117-123 of SEQ ID NO: 1) of NI-202.21D11.
Pepscan analysis revealed that NI-202.21D11 overlapped with peptides B08 (aa 109-123 of SEQ ID NO: 1), B09 (aa 113-127 of SEQ ID NO: 1) and B10 (aa of SEQ ID NO: 117-131) Binding to human α-synuclein of 1) shows that the minimal sequence required for NI-202.21D11 binding is PVDPDNE within human α-synuclein (aa 117-123 of SEQ ID NO: 1).