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Creative Biolabs

rAAV-hSyn-Con Fon-hM4D(Gi)-EGFP-WPRE-hGH polyA

[CAT#: NTA-2010-TT87] Review(5) Q&As(3)

Tracer Type:
Chemogenetics
Applications:
Chemogenetics

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Product Overview

Description

Con/Fon, expression of the hM4D(Gi) gene for inhibitory DREADD manipulation with hSyn promoter.

Tracer Type

Chemogenetics

Functional Gene

hM4D(Gi)

Activity

Inhibition

Serotype

The available serotypes are 1, 2, 5, 6, 8, 9, rh10, DJ, DJ8, PHP.B, PHP.eB, PHP.S, retro, anc80.
Novel peptide modified serotypes can also be produced on a case-by-case basis.

Virus Type

Adeno-Associated Virus (AAV)

Applications

Chemogenetics

Research Areas

Neural Circuit Mapping

Direction of Transport

Anterograde/Retrograde

Cell Types

Neuron

Promoter

Syn

Expression

Normal Expression
Properites

Titer

≥2×10^12vg/ml

Fluorophore

EGFP

Storage

Should be stored at -80°C

Research Use Only

For research use only.
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Customer Reviews and Q&As
Customer Reviews Average Customer Ratings Overall
5.0
user
Excellent
For our neural circuit mapping studies
The expression of EGFP was robust and the hM4D(Gi) functioned as expected, allowing precise control over neuronal activity. The product was easy to work with, and the WPRE element significantly enhanced transgene expression. Highly recommended for any neuroscientific research.
user
Excellent
The viral vector provided excellent transduction efficiency in our primary neuronal cultures
The fluorescent marker allowed for easy identification of transduced cells, and the DREADD receptor's functionality was confirmed through subsequent pharmacological tests. The inclusion of the hGH polyA signal appears to enhance the stability of our transgene expression. Overall, a fantastic tool for our research.
user
Excellent
This product exceeded our expectations in terms of efficiency and reliability
The hM4D(Gi)-EGFP construct was particularly useful for our optogenetic studies, providing clear visualization and control of neuronal populations.
user
Excellent
Our lab has been very impressed with the performance of this AAV vector
This AAV vector has been a vital tool in our research on learning and memory. The high transduction efficiency and neuron-specific expression were exactly what we needed. The vector's performance has been consistently excellent.
user
Excellent
Excellent product
We have used this AAV vector in several experiments, and it has consistently delivered high transduction efficiency and robust expression. The hM4D(Gi) receptor allowed for effective chemogenetic control, making our research on neural circuits much more manageable.
Q&As
Can this vector be used in non-neuronal tissues?
The rAAV-hSyn promoter is neuron-specific, which means that expression is limited predominantly to neuronal cells. While off-target expression in non-neuronal tissues is minimal, it's primarily suited for brain and neuronal tissue studies. For non-neuronal applications, consider using vectors with more suitable promoters for your target cell type.
How long does expression last after administration, and when does it peak?
Expression of the hM4D(Gi)-EGFP typically begins within a few days post-injection and reaches peak levels around two to three weeks. This timing may vary depending on the host organism and injection site. Expression can be stable for several months, allowing for long-term studies of neuronal function and behavior under chemogenetic manipulation.
Are there any known side effects or limitations in using hM4D(Gi) in chemogenetics?
While hM4D(Gi) is generally well-tolerated, potential side effects include off-target effects from the ligand (CNO) and variability in expression levels. In some cases, high expression may lead to cellular stress or toxicity. It’s crucial to optimize vector dosage and monitor animals closely for any adverse effects throughout the study.
For Research Use Only. Not For Clinical Use.
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