Human APOE Knockout HEK293T Cell Line
[CAT#: NCL2008ZP508]
Human APOE knockout cell line
- Species:
- Human
- Applications:
- Cell Assay
- Cell Types:
- HEK 293T
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
This is a human APOE gene knockout HEK293T cell line which achieved by using CRISPR/Cas9 gene editing technology.
Recommended control cell line: Human Wild Type HEK293T cell line (ab255449)
Cryopreservation cell culture medium: Cell Freezing Medium-DMSO serum-free medium supplemented with 10% (v/v) DMSO
Recommended control cell line: Human Wild Type HEK293T cell line (ab255449)
Cryopreservation cell culture medium: Cell Freezing Medium-DMSO serum-free medium supplemented with 10% (v/v) DMSO
Cell Types
HEK 293T
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 1 bp insertion in exon 3
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Species
Human
Properties
Size
1 x 10e6 cells/vial, 1 mL
Formulation
Constituents: 8.7% DMSO, 2% Cellulose, methyl ether
Cell State
Live cells are in mid log-phase growth.
Growth Pattern
Adherent
STR Analysis
Amelogenin X D5S818: 8, 9 D13S317: 12, 14 D7S820: 11 D16S539: 9, 13 vWA: 16, 19 TH01: 7, 9.3 TPOX: 11 CSF1PO: 11, 12
Biosafety Level
2
Tissue Source
Kidney
Cell Purity
>95%
Cell Viability
~90%
Antibiotic Resistance
Puromycin 1.00µg/ml
Mycoplasma Testing
Yes
Sterility Testing
Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.
Shipping
Dry ice
Storage
Shipped on Dry Ice. Store in liquid nitrogen.
Handling Advice
Upon arrival, the sample vial should be stored in liquid nitrogen vapor phase instead of -80°C. Storage at -80°C will cause loss of vitality.
1. Thaw the vial in a 37°C water bath for about 1-2 minutes.
2. Transfer the cell suspension to a 15 mL conical tube containing pre-warmed 5 mL complete medium DMEM + 10% FBS, and rotate 125 x g for about 5 minutes at room temperature.
3. Resuspend the cell pellet with 1 mL of pre-warmed complete medium DMEM + 10% FBS, and distribute it into a 25 cm2 culture flask, which contains 10 mL of pre-warmed complete medium DMEM + 10% FBS.
4. Incubate the culture with 5% CO2 in a 37°C incubator.
5. The recommended subculture ratio is 1:4-1:6. When cells grow at 80-90% confluence and divide, the cells should be passaged.
1. Thaw the vial in a 37°C water bath for about 1-2 minutes.
2. Transfer the cell suspension to a 15 mL conical tube containing pre-warmed 5 mL complete medium DMEM + 10% FBS, and rotate 125 x g for about 5 minutes at room temperature.
3. Resuspend the cell pellet with 1 mL of pre-warmed complete medium DMEM + 10% FBS, and distribute it into a 25 cm2 culture flask, which contains 10 mL of pre-warmed complete medium DMEM + 10% FBS.
4. Incubate the culture with 5% CO2 in a 37°C incubator.
5. The recommended subculture ratio is 1:4-1:6. When cells grow at 80-90% confluence and divide, the cells should be passaged.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Warnings
Store in gas phase of liquid nitrogen immediately upon receipt. This product is stable for 6 months when stored as directed.
Quality Control
Mycoplasma was tested for sterility, post-freezing viability, and short-term repeat (STR) analysis for cell line identification. Cytochrome oxidase I (COI) was analyzed for cell line species identification.
Target Details
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
