Human GBA Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP493]

Human GBA knockout cell lysate

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human GBA has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 3.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Relevant Diseases

Parkinson's Disease

Research Areas

Neurodegeneration

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8, 12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

GBA

Official Name

GBA

Full Name

Glucosylceramidase Beta

Alternative Names

Glucosylceramidase Beta; D-Glucosyl-N-Acylsphingosine Glucohydrolase; Glucosidase; Beta; Acid; Beta-Glucocerebrosidase; Acid Beta-Glucosidase; Glucosylceramidase; Imiglucerase; Alglucerase; EC 3.2.1.45;