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Creative Biolabs
Product

Human HTRA2 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP505]

Human HTRA2 knockout cell lysate

Species:
Human
Cell Types:
HEK293T

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human HTRA2 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HEK293T

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Relevant Diseases

Parkinson's Disease

Research Areas

Neurodegeneration

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

HTRA2

Official Name

HTRA2

Full Name

Serine protease HTRA2, mitochondrial

Alternative Names

HTRA2; Htra2; AI481710; Omi; Prss25; mnd2; PARK13; HtrA serine peptidase 2; MGCA8

Gene ID

27429(Human); 64704(Mouse); 297376(Rat)

Uniprot ID

O43464(Human); Q9JIY5(Mouse)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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