Creative Biolabs

Human HTRA2 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP505]

Human HTRA2 knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HEK 293T

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Description
This is a HEK293T cell transfected lysate in which Human HTRA2 has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HEK 293T
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
Passage Number
<20
Knockout Validation
Sanger Sequencing
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Pathways
Parkinson's Disease Signaling
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12
Tissue Source
Kidney
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
HTRA2
Official Name
HTRA2
Full Name
Serine protease HTRA2, mitochondrial
Alternative Names
HTRA2; Htra2; AI481710; Omi; Prss25; mnd2; PARK13; HtrA serine peptidase 2; MGCA8
Gene ID
27429(Human); 64704(Mouse); 297376(Rat)
Uniprot ID
O43464(Human); Q9JIY5(Mouse)
For Research Use Only. Not For Clinical Use.
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