Human NEFM Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP257]

Human NEFM knockout cell lysate

Species:: Human

Cell Types:: Other Cells

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Product Overview

Description

This is a HEK293T cell transfected lysate in which Human NEFM has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

Other Cells

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and Insertion of the selection cassette in exon 2.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Research Areas

Neural Cell

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

NEFM

Official Name

NEFM

Full Name

Neurofilament Medium

Alternative Names

Neurofilament Medium; Neurofilament; Medium Polypeptide 150kDa; Neurofilament Triplet M Protein; 160 KDa Neurofilament Protein; Neurofilament 3; NEF3;

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