Human PICALM Knockout HeLa Cell Lysate
[CAT#: NCL2008ZP568]
Human PICALM knockout cell lysate
- Species:
- Human
- Applications:
- Cell Assay
- Cell Types:
- HeLa
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
This is a HeLa cell transfected lysate in which Human PICALM has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HeLa
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 5 bp deletion in exon4.
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Pathways
Alzheimer's Disease Signaling
Marker
Tangles & Tau
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
PICALM
Official Name
PICALM
Full Name
Phosphatidylinositol Binding Clathrin Assembly Protein
Alternative Names
Phosphatidylinositol Binding Clathrin Assembly Protein; Clathrin Assembly Lymphoid Myeloid Leukemia Protein; CALM; Phosphatidylinositol-Binding Clathrin Assembly Protein; CLTH; LAP;
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
