Creative Biolabs

Human PICALM Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP568]

Human PICALM knockout cell lysate

Species:
Human
Applications:
Cell Assay
Cell Types:
HeLa

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Description
This is a HeLa cell transfected lysate in which Human PICALM has been knockout by using CRISPR/Cas9 gene editing technology.
Cell Types
HeLa
Cell Location
Epithelial
Applications
Cell Assay
Application Notes
To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.
Mutation Description
Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4 and 5 bp deletion in exon4.
Passage Number
<20
Knockout Validation
Sanger Sequencing, Western Blot (WB)
Relevant Diseases
Neurological Disorders
Research Areas
Neurodegeneration
Pathways
Alzheimer's Disease Signaling
Marker
Tangles & Tau
Species
Human
Cell State
Cell lysate
STR Analysis
Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10
Cell Purity
>95%
Storage
Store at -80°C. Please refer to protocols.
Research Use Only
For research use only, not for diagnostic or therapeutic use.
Target
PICALM
Official Name
PICALM
Full Name
Phosphatidylinositol Binding Clathrin Assembly Protein
Alternative Names
Phosphatidylinositol Binding Clathrin Assembly Protein; Clathrin Assembly Lymphoid Myeloid Leukemia Protein; CALM; Phosphatidylinositol-Binding Clathrin Assembly Protein; CLTH; LAP;
Gene ID
8301(Human); 233489(Mouse)
Uniprot ID
Q13492(Human); Q7M6Y3(Mouse)
For Research Use Only. Not For Clinical Use.
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