Product

Human APBB1 Knockout HEK293T Cell Lysate

[CAT#: NCL2008ZP589]

Human APBB1 knockout cell lysate

Species:: Human

Cell Types:: HEK293T

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Inquiry

Product Overview

Description

This is a HEK293T cell transfected lysate in which Human APBB1 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HEK293T

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon2.

Passage Number

<20

Knockout Validation

Sanger Sequencing

Relevant Diseases

Alzheimer's Disease

Research Areas

Neurodegeneration

Marker

Tangles & Tau

Species

Human

Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 8, 9
D13S317: 12, 14
D7S820: 11
D16S539: 9, 13
vWA: 16, 19
TH01: 7, 9.3
TPOX: 11
CSF1PO: 11, 12

Tissue Source

Kidney

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target Details

Target

FE65

Official Name

APBB1

Full Name

Amyloid beta A4 precursor protein-binding family B member 1

Alternative Names

APBB1; amyloid beta (A4) precursor protein-binding; family B; member 1 (Fe65); FE65; MGC:9072; RIR; amyloid beta precursor protein binding family B member 1