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Creative Biolabs
Product

Human PON2 Knockout HeLa Cell Lysate

[CAT#: NCL2008ZP495]

Human PON2 knockout cell lysate

Species:
Human
Cell Types:
HeLa

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Product Overview

Description

This is a HeLa cell transfected lysate in which Human PON2 has been knockout by using CRISPR/Cas9 gene editing technology.

Cell Types

HeLa

Cell Location

Epithelial

Application Notes

To use as WB control, resuspend the lyophilizate in 50 µL of LDS* Sample Buffer to have a final concentration of 2 mg/ml. For reducing conditions, we recommend a final concentration of 0.1 M DTT.

*Usage of SDS sample buffer is not recommended with these lyophilized lysates.

Mutation Description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4.

Passage Number

<20

Knockout Validation

Sanger Sequencing, Western Blot (WB)

Relevant Diseases

Parkinson's Disease

Research Areas

Neurodegeneration

Species

Human
Properties

Cell State

Cell lysate

STR Analysis

Amelogenin X
D5S818: 11, 12
D13S317: 12, 13.3
D7S820: 8, 12
D16S539: 9, 10
vWA: 16, 18
TH01: 7
TPOX: 8,12
CSF1PO: 9, 10

Cell Purity

>95%

Storage

Store at -80°C. Please refer to protocols.

Research Use Only

For research use only, not for diagnostic or therapeutic use.
Target Details

Target

PON2

Official Name

PON2

Full Name

paraoxonase 2

Alternative Names

PON2; paraoxonase 2

Gene ID

5445(Human); 330260(Mouse); 296851(Rat)

Uniprot ID

Q15165(Human); Q62086(Mouse); Q6AXM8(Rat)
Publications

Publications (0)

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For Research Use Only. Not For Clinical Use.
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