Mouse Anti-TDP43 Monoclonal Antibody (CBP6628)

Description

TDP43 was originally identified as a protein that binds to the "transactivation response" (TAR) sequence in the long terminal repeat of the HIV-1 viral genome (1). UV cross-linking of HeLa cell extracts revealed a 43kDa protein, which was cloned and sequenced, and revealed two central copies of the RNA recognition motif or RRM domain containing approximately 90 amino acids. RRM is an acronym for RNA Recognition Motif. This domain is found in many proteins that bind single-stranded RNA and some proteins that bind single-stranded DNA. It also has a single binuclear localization sequence in the N-terminal region before the first RRM domain, while the C-terminal region outside the second RRM domain is rich in glycine, asparagine, and glutamine. TDP43 is usually expressed in the central nervous system of mammals, but it accumulates in nuclear inclusions in the neurons of patients with frontotemporal degeneration (FTLD), amyotrophic lateral sclerosis (ALS), Alzheimer's disease, and other diseases (2-4). Disease-related point mutations in TDP43 are almost always found in the C-terminal region. TDP43 is related to FUS/TLS, EWSR1 and TAF15 in protein sequence, and all four proteins have a tendency, especially when they are mutated, they converge into inclusion bodies and are related to a variety of neurological diseases (5,6).

Immunogen

Full-length recombinant human TDP43 expressed and purified in E. coli.

Species Reactivity

Human; Rat; Mouse; Cow; Porcine; Horse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP6628

Applications

WB; IF; IHC

Application Notes

WB: 1:5000
ICC/IF and IHC 1:1000

Form

Liquid

Formulation

50% PBS, 50% glycerol

Preservatives

5mM NaN3

Concentration

1 mg/ml

Shipping

Gel Pack

Storage

Store at 4°C for short term, for longer term at -20°C.

Handling Advice

The antibody solution should be gently mixed before use.

Research Use Only

For research use only

Target

TARDBP

Official Name

TARDBP

Full Name

TARDBP Gene(Protein Coding) TAR DNA Binding Protein

Alternative Names

TAR DNA Binding Protein; TDP-43; TAR DNA-Binding Protein 43; TAR DNA-Binding Protein-43; ALS10; TDP43;

Gene ID

23435(Human)

Uniprot ID

Q13148(Human)