Mouse Anti-TARDBP Monoclonal Antibody (CBP2599)

Description

Transactivation response (TAR) DNA-binding protein was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 60019-2-Ig is a mouse monoclonal antibody recognizing the cleavage product of 20-30 kDa in addition to the native and phosphorylated forms of TDP-43. Immunohistochemical analyses of TDP-43 using this antibody detect both normal diffuse nuclear staining and insoluble inclusions in pathologic tissues. Notably this antibody only recognizes human TDP-43 but not reacts with mouse or rat TDP-43.

Expression Host

Mammalian cells

Immunogen

Fusion protein

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2599

Applications

FC; IHC; IP; WB; ELISA

Application Notes

IHC : 1:20-1:200; IP : 0.5-4.0 ug; WB : 1:500-1:1000

Research Areas

Neural Signal Transduction

Conjugation

Unconjugated

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1067 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

TARDBP

Official Name

TARDBP

Full Name

TAR DNA binding protein

Alternative Names

ALS10; TAR DNA binding protein; TAR DNA binding protein 43; TARDBP; TDP 43; TDP43

Gene ID

23435(Human); 230908(Mouse); 298648(Rat)

Uniprot ID

Q13148(Human); Q921F2(Mouse)

1. Sproviero, D., La Salvia, S., Colombo, F., Zucca, S., Pansarasa, O., Diamanti, L.,... & Lauranzano, E. (2019). Leukocyte derived microvesicles as disease progression biomarkers in slow progressing Amyotrophic Lateral Sclerosis patients. Frontiers in Neuroscience, 13, 344. 2. Jiang, T., Handley, E., Brizuela, M., Dawkins, E., Lewis, K. E., Clark, R. M.,... & Blizzard, C. A. (2019). Amyotrophic lateral sclerosis mutant TDP-43 may cause synaptic dysfunction through altered dendritic spine function. Disease Models & Mechanisms, 12(5). 3. Coudert, L., Nonaka, T., Bernard, E., Hasegawa, M., Schaeffer, L., & Leblanc, P. (2019). Phosphorylated and aggregated TDP-43 with seeding properties are induced upon mutant Huntingtin (mHtt) polyglutamine expression in human cellular models. Cellular and Molecular Life Sciences, 76(13), 2615-2632.