Mouse Anti-TARDBP Monoclonal Antibody (CBP2745)

Product Overview

Description

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form.

Expression Host

Mammalian cells

Immunogen

Peptide

Species Reactivity

Human; Mouse; Rat

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1

Clone Number

CBP2745

Applications

WB; ELISA

Application Notes

WB : 1:500-1:1000

Research Areas

Metabolism

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

2439 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

TARDBP

Official Name

TARDBP

Full Name

TAR DNA binding protein

Alternative Names

ALS10; TAR DNA binding protein; TAR DNA binding protein 43; TARDBP; TDP 43; TDP43

Gene ID

23435(Human); 230908(Mouse); 298648(Rat)

Uniprot ID

Q13148(Human); Q921F2(Mouse)

References

1. Sproviero, D., La Salvia, S., Colombo, F., Zucca, S., Pansarasa, O., Diamanti, L.,... & Lauranzano, E. (2019). Leukocyte derived microvesicles as disease progression biomarkers in slow progressing Amyotrophic Lateral Sclerosis patients. Frontiers in Neuroscience, 13, 344. 2. Jiang, T., Handley, E., Brizuela, M., Dawkins, E., Lewis, K. E., Clark, R. M.,... & Blizzard, C. A. (2019). Amyotrophic lateral sclerosis mutant TDP-43 may cause synaptic dysfunction through altered dendritic spine function. Disease Models & Mechanisms, 12(5). 3. Coudert, L., Nonaka, T., Bernard, E., Hasegawa, M., Schaeffer, L., & Leblanc, P. (2019). Phosphorylated and aggregated TDP-43 with seeding properties are induced upon mutant Huntingtin (mHtt) polyglutamine expression in human cellular models. Cellular and Molecular Life Sciences, 76(13), 2615-2632.

Publications