Mouse Anti-TARDBP Monoclonal Antibody (CBP2687)

Product Overview

Description

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form.

Expression Host

Mammalian cells

Immunogen

Peptide

Species Reactivity

Human; Mouse

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG2a

Clone Number

CBP2687

Applications

FC; WB; ELISA

Application Notes

WB : 1:500-1:1000

Research Areas

Neural Signal Transduction

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, 0.1% sodium azide and 50% glycerol pH 7.3.

Preservatives

Yes

Concentration

1641 μg/ml

Purification

Antigen affinity purification

Purity

>95%, as determined by SDS-PAGE

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -20°C. Stable for one year after shipment.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

TARDBP

Official Name

TARDBP

Full Name

TAR DNA binding protein

Alternative Names

ALS10; TAR DNA binding protein; TAR DNA binding protein 43; TARDBP; TDP 43; TDP43

Gene ID

23435(Human); 230908(Mouse); 298648(Rat)

Uniprot ID

Q13148(Human); Q921F2(Mouse)

References

1. Sproviero, D., La Salvia, S., Colombo, F., Zucca, S., Pansarasa, O., Diamanti, L.,... & Lauranzano, E. (2019). Leukocyte derived microvesicles as disease progression biomarkers in slow progressing Amyotrophic Lateral Sclerosis patients. Frontiers in Neuroscience, 13, 344. 2. Jiang, T., Handley, E., Brizuela, M., Dawkins, E., Lewis, K. E., Clark, R. M.,... & Blizzard, C. A. (2019). Amyotrophic lateral sclerosis mutant TDP-43 may cause synaptic dysfunction through altered dendritic spine function. Disease Models & Mechanisms, 12(5). 3. Coudert, L., Nonaka, T., Bernard, E., Hasegawa, M., Schaeffer, L., & Leblanc, P. (2019). Phosphorylated and aggregated TDP-43 with seeding properties are induced upon mutant Huntingtin (mHtt) polyglutamine expression in human cellular models. Cellular and Molecular Life Sciences, 76(13), 2615-2632.

Publications