Rat RN33B Cells

[CAT#: NCB2108FY0061]

Animal cells

Species:: Rat

Applications:: 3D Cell Culture

Cell Types:: Schwann Cells

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Product Overview

Description

Incubate the culture at 33°C in a suitable incubator. A 95% Air, 5% CO2 in air atmosphere is recommended if using the medium described on this product.

Cell Types

Schwann Cells

Applications

3D Cell Culture

Application Notes

The cells can be used as a model of neuronal differentiation in vitro.
Immunohistochemical and Northern blot analysis revealed high levels of low affinity NGF receptor protein and mRNA in differentiated RN33B cells.

Research Areas

Neural Cell

Species

Rat

Expression Profile

enolase; nestin; neurofilament; vimentin

Properties

Form

Frozen

Growth Pattern

Adherent

Morphology

Spindle-Shaped At 33C; Neurite-Like At 37C

Immortalization Method

SV40 large T antigen transformed

Culture Medium

The base medium for this cell line is DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Tissue Source

Brain; Medullary raphe nucleus

Age

12.5 days

Strain

Sprague-Dawley

Shipping

Dry ice

Storage

Vapor phase of liquid nitrogen

Handling Advice

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.