Rat RN33B Cells
[CAT#: NCB2108FY0061]
Animal cells
- Species:
- Rat
- Applications:
- 3D Cell Culture
- Cell Types:
- Schwann Cell
Certificate of Analysis Lookup
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Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Product Overview
Description
Incubate the culture at 33°C in a suitable incubator. A 95% Air, 5% CO2 in air atmosphere is recommended if using the medium described on this product.
Cell Types
Schwann Cell
Applications
3D Cell Culture
Application Notes
The cells can be used as a model of neuronal differentiation in vitro.
Immunohistochemical and Northern blot analysis revealed high levels of low affinity NGF receptor protein and mRNA in differentiated RN33B cells.
Immunohistochemical and Northern blot analysis revealed high levels of low affinity NGF receptor protein and mRNA in differentiated RN33B cells.
Research Areas
Neural Cell
Species
Rat
Expression Profile
enolase; nestin; neurofilament; vimentin
Properties
Form
Frozen
Growth Pattern
Adherent
Morphology
Spindle-Shaped At 33C; Neurite-Like At 37C
Immortalization Method
SV40 large T antigen transformed
Culture Medium
The base medium for this cell line is DMEM:F12 Medium Catalog No. 30-2006. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Tissue Source
Brain; Medullary raphe nucleus
Age
12.5 days
Strain
Sprague-Dawley
Shipping
Dry ice
Storage
Vapor phase of liquid nitrogen
Handling Advice
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.
Research Use Only
For research use only
Neural Cell Culture
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
