C57BL6 Mouse Schwann Cells, Immortalized
[CAT#: NCL-2105-P168-AM]
These cells can be used for disease modeling, drug screening and toxicology testing.
- Species:
- Mouse
- Applications:
- Cell Assay
- Cell Types:
- Schwann Cell
Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number
Description
A spontaneously immortal wild-type Schwann cell line was established from the dorsal root ganglia (DRG) of C57BL6 mice and adjacent peripheral nerves. Immortalized C57BL6 mouse Schwann cells retain the characteristics of Schwann cells, such as the production and secretion of various neurotrophic factors and cytokines (NGF and GDNF) that promote neuron survival.
Cell Types
Schwann Cell
Applications
Cell Assay
Application Notes
The basic medium for this cell line is PriNeu III medium provided by Creative Biolabs, catalog number. NCL-2105-P169-AM. To make a complete growth medium, please add the following ingredients to the basic medium: Fetal Bovine Serum (NCL -2105-P170-AM)* Final concentration is 5%, recombinant human NRG1 (NCL-2105-P171-AM) final concentration is 20 ng/mL, penicillin/streptomycin solution (NCL-2105-P172- AM ) to a final concentration of 1%.
Change the media every 2-3 days.
Carbon dioxide (CO2): 5%, temperature: 37.0
* Unless otherwise specified, do not use heat-inactivated FBS for cell culture.
Change the media every 2-3 days.
Carbon dioxide (CO2): 5%, temperature: 37.0
* Unless otherwise specified, do not use heat-inactivated FBS for cell culture.
Species
Mouse
Freeze Medium
90% FBS and 10% DMSO
Tissue Source
Brain
Cell Purity
>95%
Cell Viability
>90%
Mycoplasma Testing
Negative
Sterility Testing
The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.
Genetic Stability Testing
We perform cell genetic stability studies under ICH guidelines. We can provide guidance on the appropriate testing program upon your requirements.
Shipping
Frozen on dry ice
Storage
Liquid nitrogen immediately upon delivery
Handling Advice
Frozen cells should be thawed immediately upon receipt and grown according to handling procedure to ensure cell viability and proper assay performance.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Note: Do not freeze the cells upon receipt as it may result in irreversible damage to the cell line.
Research Use Only
For research use only
Quality Control
1) Immunofluorescence
Neural Cell Lines
For Research Use Only. Not For Clinical Use.
