Rat RT4-D6P2T Cells

[CAT#: NCB2108FY0111]

Animal cells

Species:: Rat

Applications:: 3D Cell Culture

Cell Types:: Schwann Cell

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Product Overview

Description

Incubate the culture at 37°C in a suitable incubator. A 95% Air, 5% CO2 in air atmosphere is recommended if using the medium described on this product.

Cell Types

Schwann Cell

Applications

3D Cell Culture

Application Notes

This cell line is a suitable transfection host.

Relevant Diseases

Schwannoma

Research Areas

Neural Cell

Species

Rat

Properties

Form

Frozen

Growth Pattern

Adherent

Morphology

Neuronal

Culture Medium

The base medium for this cell line is Dulbecco's modified Eagle's medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Freeze Medium

Complete growth medium supplemented with 5% (v/v) DMSO

Tissue Source

Peripheral nervous system

Shipping

Dry ice

Storage

Vapor phase of liquid nitrogen

Handling Advice

Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130°C, preferably in liquid nitrogen vapor, until ready for use.

Research Use Only

For research use only

Donor Information

Additional Donor Information

RT4-D6P2T is a schwannoma cell line derived from N-ethyl-N-nitrosourea (ENU)-induced rat peripheral nerve tumor RT4. The tumor grows in culture, and the cell line RT4-AC is isolated, and then develops in a different cell type RT4-D to produce glial-specific S100. RT4-D was subcloned to produce RT4-D6, RT4-D6 was subcloned to produce RT-4-D6P2, and then finally subcloned to produce D6P2T subclone.

Publications