Neural Stem Cell Differentiate Service

Stem cells are considered an ideal source for tissue regeneration therapy due to their stem cell properties and regenerative capacity, and because of their ability to differentiate into neural cells under specific conditions, stem cells are also regarded as an important tool for neurobiological research. Creative Biolabs provides customers with comprehensive primary stem cell extraction, isolation, and neural stem cell differentiation services.

Stem Cells for Neuron Differentiation

Stem cells are highly stemness and regenerative, which means that they can renew and generate progenitor cells and further differentiate into specific cell types. They are important cell sources for tissue regeneration therapy and neural network research. In the research of neural cell differentiation, the most commonly used cell types are mesenchymal stem cells (MSCs) and neural stem cells (NSCs).

Fig 1. Induced differentiated NSCs in vitro. (Labeed, 2011)

MSCs

MSCs are attractive sources for stem cell therapy, which can be easily obtained from peripheral blood without ethical concerns or tumorigenesis. MSCs cultured under different matrix materials or stimulated by specific chemical molecules can differentiate into various neural cells, such as retinal cells, retinal organoids, oligodendrocytes, and central nervous system cells.

NSCs

NSCs are tripotent stem cells that, after generating precursor cells, are restricted to three lineages: neurons, oligodendrocytes, and astrocytes. Neuronal progenitor cells can be further differentiated into various neuronal cells.

Fig 2. Neural stem cells and major transcription factors. (Park, 2018)

Differentiation of NSCs

Under different culture conditions or biochemical molecular stimulation, NSCs will differentiate into different types of neural cells. With years of experience in the field of neuroscience, Creative Biolabs isolates and extracts primary NSCs for your project from a variety of sources, induces and differentiates neural cells in vitro according to your needs, and establishes a repeatable and stable experimental platform. In addition, NSCs induced by iPSCs are also an ideal and easy-to-obtain cell source.

Optimized induction and culture protocols allow Creative Biolabs to provide our customers with high-quality and high-purity differentiated neural cell lines. You may choose between a variety of neural precursor cells or neuronal cells with mature functions, customize your experimental protocol, and construct suitable in vitro research models. The differentiated nerve cells we provide include but are not limited to astrocytes, oligodendrocytes, dopaminergic neurons, sensory neurons, motor neurons, glutamatergic neurons, and various precursor cells. These well-defined cell lines, which recapitulate the physiology of primary cells while allowing you to maintain long-term in vitro culture, will be the best tools for your neurophysiology and pathology research.

Fig 3. iPSC and NSC induction differentiation route.

Characterization of Differentiated NSCs

In addition to well-characterized differentiated neural cells, Creative Biolabs also provides additional characterization services. You can use your cells or suitable differentiated/primary cells provided by us for antibody staining. By tracking and measuring markers on the surface of nerve cells, we can label those cells at specific stages of differentiation and analyze their biological properties and neurogenic potential. Cells and their surface markers commonly used in neural cell lines are listed below.

In addition, we also assist you to implement any downstream analytical testing you need to advance your experiments. We detect and characterize stem cell proliferation and differentiation in vitro by a variety of typical biochemical assays, such as reverse transcription polymerase chain reaction, immunocytochemistry, flow cytometry, or various blotting tests. We can also help you complete more advanced downstream analysis such as global transcriptome analysis, neurite length analysis, MEA (multi-electrode array) assay, and one-stop neurological disease study solution and provide you with stable, repeatable, and comprehensive degrees.

Fig 4. Morphological and key protein staining analysis of differentiated NSCs. (Shi, 2022)

Through years of continuous learning and optimization, Creative Biolabs has developed a stable neural cell production process. Not only can differently types of nerve cells be provided in large and excellent quantities, but also customized production services can be offered and developed according to your needs. Our stable and reliable neural stem cell differentiation services will be a powerful addition to your neurodegenerative disease and neurology research. Please do not hesitate to contact us for more information.

References

1. Labeed, F.H.; et al. Biophysical characteristics reveal neural stem cell differentiation potential. PLoS ONE. 2011, 6(9): e25458.
2. Park, S.Y.; et al. Phospholipase D1 signaling: essential roles in neural stem cell differentiation. Journal of Molecular Neuroscience. 2018, 64: 333-340.
3. Shi, B.; et al. Chiral nanoparticles force neural stem cell differentiation to alleviate Alzheimer’s disease. Advanced Science. 2022, 9: 2202475.