Neurons Electrophysiology Assay

Neuroelectrophysiology is a method of recording or measuring electrical properties, membrane potential conduction velocity, and ion channel activity in whole animals or isolated organ tissues, nerves, and, cellular ion channels using electrophysiological instruments, microelectrodes, voltage clamp, and patch clamp techniques. Electrical properties of neural tissue are important in various applications such as therapeutic electrical stimulation of the nervous system, electrical impedance tomography (EIT), and interpretation of intrinsic electrical signals in neuroscience.

At Creative Biolabs, we provide neuron electrophysiological assay service for our global clients, facilitating your study of neuropathophysiology, pharmacology, and signal transduction processes at the cellular and molecular levels.

Why Neurons Electrophysiology Assay is Needed?

In neuroscience research, the brain, the most important organ, is a spectacularly complex electrochemical computing machine with many components (neurons) and interconnections (synapses). Action potentials are important features of normal neuronal function

Lesions of brain components have caused a variety of neurological disorders. Neuroimaging methods, such as structural/functional magnetic resonance imaging (MRI) and positron emission tomography, (PET) have certain advantages that include excellent spatial resolution; yet they offer relatively poor temporal resolution compared to electrophysiological methods that evaluate brain function in the millisecond range.

Electrophysiology provides some good measurements of the acute and chronic effects of neurological diseases on the brain. It plays a critical role in the understanding of pathological mechanisms, the study of signal transduction and cytokine signaling pathways, and the development of new drugs.

Neurons Electrophysiology Applications

• In researches

Over the years, numerous studies have been conducted using neuron electrophysiology, involving multiple research directions.

Fig. 1 LTP in PV+ interneurons are impaired in γCaMKII PV-KO mice. (He, et al., 2021)

By combining electrophysiological, molecular, and cellular studies with a multidisciplinary analysis of genetically engineered mice, γCaMKII was found to be the key molecule that neuroscientists have sought for many years to regulate the long-term plasticity (LTP) of PV-inhibitory neurons.

Fig. 2 Oxytocin reduces the maintenance of pre-LTP. (Li, et al., 2021)

The whole-cell patch clamp was used to record the effects of Oxytocin on pre-LTP and post-LTP. Experimental results suggest that Oxytocin can selectively eliminate anxiety behaviors caused by chronic pain.

• In disease
• Abnormal visual evoked potentials (VEP) in multiple sclerosis (MS) and AIDS.
• Objective assessment of color blindness, visual field defects, and visual impairment in infants.
• Assessment of epilepsy and stroke.
• Cerebral oxygen metabolism in brain injury and mild hypothermia.
• Lesion assessment of the peripheral nervous system in amyotrophic lateral sclerosis (ALS).
• Diagnosis of diabetic peripheral neuropathy.
• Recognition of cognitive impairment in Parkinson's disease.

Neuroelectrophysiology has been widely used in studies of multiple diseases for the exploration of pathological mechanisms and cytokine functions, even the development of new drugs.

Creative Biolabs has mature biological research technology and experienced technicians. In the past 20 years, we have provided a variety of satisfactory services to scientific researchers worldwide. Here, we would like to emphasize our high professionalism in neuron electrophysiology experiments. We have a leading experimental platform and the most advanced technology. We are also willing to answer your questions and provide high-quality customized services in neuron electrophysiology. Please contact us for more technical support.

References

1. He, X.Z.; et al. Gating of hippocampal rhythms and memory by synaptic plasticity in inhibitory interneurons. Neuron. 2021, 109(6), 1013-1028.
2. Li, X.H.; et al. Oxytocin in the anterior cingulate cortex attenuates neuropathic pain and emotional anxiety by inhibiting presynaptic long-term potentiation. Cell reports. 2021, 36(3), 109411.