Nerve Fiber Morphometric Assay Service

The nerve fiber morphometric assay is a technique that evaluates the structural and functional status of the nervous system by measuring the morphological characteristics of nerve fibers. With this information, researchers gain insights into nerve fiber degeneration, regeneration, degree of myelination, and axon diameter. To learn more about our products and services, submit a project request, or request a quote, please contact us today.

Staining Products for Nerve Fibers

In addition to our services, we continue to provide low-cost, high-quality nerve fiber staining products for your nerve fibers. Our staining products include a variety of methods, such as modified Bielschowsky staining and silver-plating staining. These methods deposit silver ions on the nerve fibers, making the nerve fibers appear dark brown or black, which is convenient for observation and research.

What Can We Provide?

We provide comprehensive services tailored to meet the unique requirements of our clients. Our team of experts is equipped to collaborate with clients to ensure robust experimental design and effectively communicate results.

• Staining and Labeling

We offer a variety of staining techniques. For example, specific staining methods (such as Azure II/Methyl Blue/Violet staining) can enhance the visualization of nerve fibers and facilitate subsequent morphological analysis. In addition, fluorescent tracing technology is often used to follow the projection path of nerve fibers.

• Microscope Imaging

We offer optical and electron microscopy services for nerve fiber observations Optical microscopes can provide a broad view, while electron microscopes can reveal more subtle structural changes.

Fig.1 Representative TEM images of sham-operated rats and rats with sciatic nerve crush injury.¹

• Auto Image Processing and Analysis

Modern nerve fiber morphometry relies on advanced image processing software. For example, using ImageJ or Volocity software, researchers can accurately separate and measure nerve fibers through steps such as automatic thresholding, expansion and contraction operations, and Boolean operations.

• Cell Quantification and Analysis

This part of the test includes microscopic fluorescence quantification and flow cytometry, which can quantitatively analyze the chemicals in cells or tissues, providing important data on nerve fiber density and distribution.

Applications

Nerve fiber morphology can help researchers understand the structure and function of the nervous system, especially in the areas of disease diagnosis, pathological mechanism research, and regenerative medicine.

At Creative Biolabs, our nerve fiber morphometric service employs dependable staining methods and cutting-edge image analysis tools and software to precisely measure and classify nerve fibers. We invite you to contact us to discuss your project plans in depth.

Reference

1. Wang, Chau-Zen, et al. "Low-level laser irradiation improves functional recovery and nerve regeneration in sciatic nerve crush rat injury model." PloS one 9.8 (2014): e103348. Distributed under Open Access license CC BY 4.0, without modification.