Service

Nerve Fiber Morphometric Assay

Nerve fiber morphology refers to the quantitative measurement of the morphological characteristics of nerve fibers, which is an invaluable tool for assessing changes in the peripheral nervous system (PNS) and central nervous system (CNS). Creative Biolabs' Nerve Fiber Morphometric Services enable researchers to assess the morphology of nerve fibers in a variety of tissues, including skin, muscle and nerves.

Nerve Fiber Morphometry Assay Services

Our nerve fiber morphometry services include the use of various techniques such as electron microscopy, light microscopy and confocal microscopy to visualize nerve fibers. We also use specialized software to measure various parameters such as axon diameter, myelin thickness, and fiber diameter.

We also offer customized nerve fiber morphometric services to meet the specific needs of our clients. Our team of experts can work with clients to develop assays tailored to their specific research needs.

Fig.1 Morphometric analysis. (Ziago, 2017)

Assay Workflow

The Nerve Fiber Morphometric Assay consists of several key steps, including tissue preparation, staining, imaging, and computer-assisted analysis.

Tissue Preparation

This step involves the dissection and processing of the tissue of interest.The tissue is embedded in paraffin, sectioned, and mounted on slides for further analysis.

Stainning

The next step involves staining the tissue section with a nerve-specific marker. The mostcommonly used markers are Protein Gene Product9.5 (PGP 9.5) and S100 protein. These markers stain the axons andSchwann cells, respectively, allowing for the identification of nerve fibers.

Imaging

The stained tissue sectionis imaged using ahigh-resolution microscope.The images are captured using a digital camera and stored for further analysis.

Data Analysis and Interpretation

The stained tissue sectionis imaged using ahigh-resolution microscopeThe images are captured using a digital camera and stored for further analysis.

Fig.2 General workflow.

Applications

The Nerve Fiber Morphometric Assay Service has broad applications in both basic and clinical research. Some of the key applications of this service include:

Evaluating changes in nerve fiber morphology associated with different diseases and conditions.
Investigating the effects of drugs and other therapeutic interventions on nerve fiber morphology.
Studying the development of nerve fibers during embryonic and postnatal development.
Characterizing nerve fiber regeneration in injured tissues.

Our Advantages

Expertise

Our team of experts has extensive experience in neuroscience and can provide you with expert advice and guidance throughout the assay process.

Flexibility

We can customize our assays to meet your specific needs and requirements, including compound type, exposure duration, and endpoint measurements.

Quality

We use state-of-the-art equipment and techniques to ensure high-quality, reliable data.

In conclusion, the Nerve Fiber Morphometric Assay Service provided by Creative Biolabs is a valuable tool for investigating changes in nerve fiber morphology. We are committed to providing our clients with the most accurate, reliable, and timely results, with a focus on patient comfort and affordability. Contact us today to learn more about our services and how we can help you.

Reference

Ziago, Eduardo Keiske Mastuda, et al. "Analysis of the variation in low-level laser energy density on the crushed sciatic nerves of rats: a morphological, quantitative, and morphometric study." Lasers in medical science 32 (2017): 369-378.

For Research Use Only. Not For Clinical Use.