Neurotoxicity Assay Service

In vitro neurotoxicity assay is a technique used to evaluate the potential deleterious effects of chemicals, pharmaceuticals, or other agents on the nervous system. At Creative Biolabs, we offer our neurotoxicity assay in a variety of models, including primary neuronal cells, neuronal cell lines, and neurons derived from induced pluripotent stem cells (iPSCs). We are committed to providing the highest standards of professional neuronal toxicity assay to our clients worldwide. To learn more about our products and services, submit a project inquiry, or request a quote, please contact us.

Neuronal Toxicity Assay Products

In addition to our services, we offer a comprehensive product line to support your in vitro neurotoxicity testing.

Introduction

Currently, in vitro neurotoxicity assay mainly relies on experimental methods such as cell culture and tissue culture and examines the effects of toxicant exposure on the structure or function of the nervous system using biochemical, molecular, electrophysiological, morphological, and various omics technologies. This detection method offers significant advantages over traditional in vivo animal experiments, including reduced costs, a shorter timeline, and higher throughput. The development of in vitro neurotoxicity detection technology not only improves research efficiency but also reduces dependence on animals, making it an important technology with a wide range of potential applications.

Neuronal Toxicity Assay

• Neurite Outgrowth Assay

Neurite outgrowth assays can provide important information about neuronal function, particularly in the study of neurodevelopment, neurodegenerative diseases, and neuroplasticity. In these assays, neurites are quantitatively analyzed along parameters such as the length of neurite outgrowths, the number of branches, and the speed of neurite development. From these assays, researchers evaluate the effects of compounds on neuronal development and identify potential therapeutic targets.

Fig.1 β-Hydroxy-β-methylbutyrate (HMB) induces neurite outgrowth in Neuro2a cells.1 A) Cell viability was measured by incubating Neuro2A cells with 25 μM HMB for 72 hours. B) Neurite outgrowth was analyzed after 48 hours of incubation with HMB. Cell morphology was observed by light microscopy (200x).

• Cytotoxicity Assay

Cytotoxicity assay is an important means to evaluate the toxic effects of compounds or drugs on cells, and is widely used in drug screening, biocompatibility testing and other fields. Commonly used cytotoxicity detection methods include the MTT method, CCK-8 method, LDH method, etc. These methods use different principles to evaluate cell viability and survival rate, proliferation ability, mitochondrial function, cell membrane integrity and other indicators.

Fig.2 Increased levels of cell death after 24 hours of treatment with toxic stimuli. Verification of staining using near-infrared Cytotox dye (blue) and propidium iodide (red) in the Live-Cell Imaging System.

• Calcium Assay

Calcium assays provide a sensitive and effective tool in cytotoxicity studies by monitoring changes in intracellular calcium concentrations to predict cellular responses to various treatments.

• Microelectrode Array (MEA) Assay

MEA arrays are capable of measuring the electrical activity of neuronal cell culture. It provides non-invasive, label-free, high-throughput assessments, making it suitable for toxicity assessment of neural networks.

• Neurons High-Content Assay

By evaluating synapse formation and cell viability of neurons using automated fluorescence imaging technology, the effects of drugs on multiple cellular toxicity indicators can be monitored and analyzed simultaneously, providing insights into the mechanism of drug toxicity.

At Creative Biolabs, our neuronal toxicity assay offers a range of benefits, including ethical and cost-effective solutions that provide precise and comprehensive data, accelerating the process of drug development and chemical safety assessment. We invite you to contact us to discuss your project plan in detail.

Reference

1. Salto, Rafael, et al. "β-Hydroxy-β-methylbutyrate (HMB) promotes neurite outgrowth in Neuro2a cells." PloS one 10.8 (2015): e0135614. Distributed under Open Access license CC BY 4.0, without modification.