T-type Calcium Channel Assay

Creative Biolabs has been working in the neurological research field for a long term. Our experienced team assists global researchers to explore the mechanisms of neurological disorders and develop novel effective therapeutics. We have established neurological discovery and development platforms for your research. T-type calcium channel assay by Creative Biolabs helps you to understand the mechanism of neuropathic pain and therefore develop new drugs.

Neuropathic Pain

Neuropathic pain appears to be related to the sensitization of signal transduction after tissue inflammation or injury. T-type calcium channels, in particular the Cav3.2 isoform, contribute to the communication and excitability of neurons all along the ascending and descending pain pathways, which are associated with primary afferent neurons, dorsal root ganglia (DRGs), and within pain-processing neurons in the central nervous system. DRG neurons, especially rat DRG neurons are well suited for studying the conduction of calcium signals in T-type calcium channels.

T-type Calcium Channel Assay

T-type calcium channel assay is used to detect how compounds affect calcium signals in neurons, especially DRG neurons. Creative Biolabs is dedicated to providing a full range of related services for the in vitro T-type calcium channel assay, including the rat primary DRG neuron cell line, advanced high-throughput technologies, and data analysis. We use dependent inhibition of T-type calcium channel assay to obtain a dose-response curve to determine the dose effects and the inhibition rate of compounds on calcium signals. Besides, our customized services can meet your research requirements in calcium signal detection to study different types of calcium channels.

Fig.1 Summary of the effect of DPBPs on T-type calcium channels. (Santi, 2002)

Applications

T-type calcium channel assay can be employed to discover how injury and different doses of different compounds affect individual T-type calcium channels in generating Ca2+ signals. Therefore, it can be used to determine the effect of compound effects on the Ca2+ signal, which is used to screen drugs for neuropathic pain, such as trigeminal neuralgia.

Our Services

Despite decades of exploration, many neurological diseases have been not completely defined. The mechanism is awaited to be explored. Creative Biolabs is committed to providing in vitro cell viability and toxicity assays, such as T-type calcium channel assay. Besides, we also offer in vivo rodent models of inflammation, injury, and chronic pain, which help researchers to comprehensively discover the mechanism of neuropathic pain and discover potential novel compounds for treatment. Please feel free to contact us for more detailed information on T-type calcium channel assay.

Reference

1. Santi, C. M.; et al. Differential inhibition of T-type calcium channels by neuroleptics. Journal of Neuroscience. 2002, 22(2): 396-403.