Central/Peripheral Neuronal Firing Assay
The study of neuronal firing patterns is critical to understanding the underlying mechanisms of neurological diseases and drug development. Central and peripheral neuronal firing assays are widely used to investigate the electrical activity of neurons in the brain and nervous system. Creative Biolabs is a leading provider of advanced neurobiology services and has extensive expertise in conducting central and peripheral neuronal firing assays using cutting-edge technologies.
Our Assays Capabilities
We offer a set of reliable and reproducible translational, phenotypic neuronal assays to demonstrate the efficacy, selectivity, potency and neurotoxic liability of neurological therapeutic compounds. By employing native rodent and human iPSCs from the central nervous system (CNS) and peripheral nervous system (PNS), our neuronal firing assays can be used for determining the efficacy, potency and mechanism-of-action of client compounds designed to treat CNS/PNS diseases.
We primarily focus on electrophysiological readouts, using manual patch (voltage and current clamp) and multielectrode array (MEA) platforms to record changes in the activity of single cells and neuronal networks.
Please click the buttons below to explore more information our our electrophysiology platforms.
Fig.1 Firing types of neurons in the central lateral amygdala. (Hunt, 2017)
Current Clamp Recording Platform
Creative Biolabs has extensive expertise in current clamp recording and can perform this technique using whole-cell recording. Our team of experts can provide detailed analysis of the recorded data and generate comprehensive reports to aid in the interpretation of the results. Please click the link above to find more information.
Multi-electrode Array Platform
Multi-electrode array recording is a high-throughput technique for measuring the electrical activity of neurons in a population. This method involves placing a grid of electrodes on the surface of the brain tissue or peripheral nerves to measure the electrical activity of a group of neurons. This technique provides spatial and temporal resolution and is essential in understanding the properties of neural networks.
Human iPSC-derived Neurons
Creative Biolabs uses human induced pluripotent stem cell (iPSC)-derived neurons for our neuronal firing assays. Human iPSC-derived neurons have several advantages over traditional animal models, including improved relevance to human biology and the ability to produce large quantities of neurons for high-throughput assays.
Fig.2 Different spatiotemporal organization of spiking activity in networks with identical mean firing rates. (McCready, 2022)
In summary, central and peripheral neuronal firing assays are valuable tools for studying the mechanisms of neurological disorders and for drug discovery. Creative Biolabs provides state-of-the-art services in the field of neuroscience, including central and peripheral neuronal firing assays using current clamp recording, multi-electrode array, and patch clamp recording techniques. We use human iPSC-derived neurons for our assays, providing improved relevance to human biology and the ability to produce large quantities of neurons for high-throughput assays.
We are committed to providing personalized and comprehensive support to our clients, from assay development and optimization to data analysis and interpretation. Get in touch with us today to find out more about how we can support you in your endeavors!
References
- Hunt, Sarah, et al. "Intrinsic circuits in the lateral central amygdala." Eneuro 4.1 (2017).
- McCready, Fraser P., et al. "Multielectrode arrays for functional phenotyping of neurons from induced pluripotent stem cell models of neurodevelopmental disorders." Biology 11.2 (2022): 316.
