Neurons High-Content Assay

What's HCA?

High-content analysis (HCA) is the process of extracting high-level information (by quantifying complex attributes) from images of cells and tissues using a combination of automated microscopy and automated image analysis. Modern neuroscience research uses many advanced imaging methods. High content analysis (HCA) is the most exciting recent development in the imaging and analysis field. Creative Biolabs' neuronal products are widely used in HCA, high-content screening, and automated image analysis. In addition, we are also willing to provide neurons HCA service to neuroscientists worldwide.

Advantages of HCA

• Standardization

The images must be acquired in a standardized way. In particular, when acquiring fluorescent images the exposure time and the number of exposures must be controlled and standardized. Creative Biolabs' technicians will set optimal parameters for segmenting and measuring objects in images to ensure all images are output in a standardized, objective manner.

• Speed

Creative Biolabs' HCA platform enables real-time image acquisition and analysis, rather than capturing images and then offline analysis. This greatly increases the throughput rate. For your research, the faster the results are obtained, the sooner valid conclusions can be made and follow-on experiments planned.

• Accuracy/Validity

As algorithms and hardware become more sophisticated, accuracy might increase to the point at which HCA becomes more accurate than even the human eye. Furthermore, Creative Biolabs' HCA platform is possible to apply multiple different assays to the same image data set and determine the level of consistency in the results.

• Image Processing

At Creative Biolabs, a major advantage of our HCA service is that powerful image-processing algorithms can be applied, to enhance signal-to-noise ratios and extract image features for more precise and accurate segmentation and analysis.

• Other Advantages

Creative Biolabs' HCA platform can integrate information from several channels, more than one cell attribute can be measured in each image. This multiplexing allows for much more sophistication in data and image analysis and interpretation and generates much 'richer' cellular information than manual methods.

In summary, Creative Biolabs provides a fast, accurate, and objective way to quantify complex information from neurons through HCA.

Fig.1 Multiple assays of neurons. ATF2 (Red), Hoechst (Green), MAP2 (Blue), triple-labeled cells (Purple). (Dragunow, 2008)

Applications of Neurons HCA

• Proliferation
• Apoptosis
• Cell counting
• Cell migration
• Neurite outgrowth
• Inclusion formation
• Signal transduction
• Gap-junction quantification
• Neurogenesis, synaptogenesis, and capillary formation

For nearly two decades, Creative Biolabs has been dedicated to cutting-edge bioscience. We have provided a variety of biomaterials and custom services to global bioscientists. Our advanced technology and quality services have also been well received by clients around the world. If you are interested in our neuron high-content assay technology and related services, please contact us for more information.

Reference

1. Dragunow, M. High-content analysis in neuroscience. Nature Reviews Neuroscience. 2008, 9(10): 779-788.