Neuronal Death Assay Service

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Introduction

Fig.1 Neuronal Death.¹

A key feature of neurodegenerative diseases is the progressive degeneration and death of neurons, resulting in cognitive dysfunction and other neurological symptoms. This pathology has been observed in nearly every neurodegenerative disease, including Alzheimer's disease (AD), Parkinson's disease (PD), and Huntington's disease (HD).

The mechanisms of neuronal death in neurodegenerative diseases are complex and diverse, mainly including oxidative stress, programmed cell necrosis, and autophagy dysfunction. Neuronal death is the core link in the development of neurodegenerative diseases, and understanding its specific mechanisms is of great importance for the development of new treatments.

Neuronal Death Assays

• Fluoro-Jade B Histofluorescence Assay

This is a histochemical method used to detect neuronal and glial cell death. Fluoro-Jade B stains all degenerating neurons but does not stain normal, healthy brain tissue. We offer a one-stop histofluorescence service with high-quality images, reliable quantification results, in-depth project reports, and quick turnaround times.

Fig.2 Detection of cell death using Fluoro-Jade B.²

• Lactate Dehydrogenase (LDH) Release Assay

When the plasma membrane of a cell is damaged, lactate dehydrogenase (LDH) is released, making it an excellent marker for cell death. This release can be utilized to quantify cell viability and cell death in vitro and in vivo.

• Annexin V-FITC/PI Dual Stain Assay

Annexin V-FITC/PI double staining is a technique used to identify different types of cell death, including apoptosis and necrosis, in neuronal cells. The combination of Annexin V-FITC and Propidium iodide (PI) allows researchers to distinguish three types of cells:

Live cells: Annexin V negative and PI negative

Early apoptotic cells: Annexin V positive, PI negative

Necrotic cells: Annexin V positive, PI positive

Fig.3 Morphological photomicrographs of Annexin V-FITC/PI assay.³

• Caspase Activity Assay

Caspase-3 is a caspase that belongs to the protease family and is one of the executioners of apoptosis. In addition, caspase-3 is not only involved in neuronal apoptosis but also related to synaptic plasticity. By detecting the relative activity of caspase, this method is also used to evaluate apoptosis.

• Custom Assays

At Creative Biolabs, we stay up to date with the latest scientific developments and are more than prepared to tailor our available neuronal death assays to your precise requirements. Furthermore, we provide high-quality materials, data, and insights to ensure the success of your project. Please contact us to discuss your project plans, and our expert scientists will be pleased to assist you in accelerating your research.

Neuronal Death-Related Products

Tools for measuring neuronal death are essential for monitoring the effects of factors, allowing for the assessment of potential treatments and the identification of optimal strategies for addressing neuronal death. We provide a comprehensive range of products to assist you in evaluating strategies for neuronal death.

References

1. Kristiansen, M., and J. Ham. "Programmed cell death during neuronal development: the sympathetic neuron model." Cell Death & Differentiation 21.7 (2014): 1025-1035. Distributed under Open Access license CC BY 3.0.
2. Tanaka, Sachiko, et al. "Activation of microglia induces symptoms of Parkinson's disease in wild-type, but not in IL-1 knockout mice." Journal of neuroinflammation 10 (2013): 1-11. Distributed under Open Access license CC BY 2.0, without modification.
3. Liu, Di, et al. "Neuroprotective effects of ginsenoside Rb1 on high glucose-induced neurotoxicity in primary cultured rat hippocampal neurons." PLoS One 8.11 (2013): e79399. Distributed under Open Access license CC BY 4.0, without modification.