Neuronal Death Assay

Neuronal cell death in the central nervous system (CNS) is considered to be a complex process to decipher. Cell death in specific regions of the brain represents a fundamental pathological feature of different neurodegenerative diseases. Given the emergence of common pathological mechanisms among diverse neurodegenerative diseases, understanding the dynamics of cell death in a specific disease can help to explore molecular mechanisms and potential therapeutic avenues for other neurological diseases. As an expert in the field of neuroscience, Creative Biolabs is committed to providing professional neuronal death assay services to customers around the world.

Neuronal Death in CNS Diseases

Neuronal cell death is a common phenomenon in nervous system development and pathology, which is closely related to a diversity of neurodegenerative diseases. Exploring the mechanism of neuronal death is an important approach to protect neurons from adverse environmental conditions. In addition, due to the limited ability of neurons to proliferate or be replaced, it is emerging as a hotspot to investigate neuronal cell death. Traditionally, neuronal cell death in CNS disease was thought to be the result of apoptosis-induced cellular stress. In fact, scientists have found that neuron death results from a variety of factors. Understanding neuronal death pathways and molecular mechanisms in the context of different diseases offers new perspectives for developing potential therapeutic strategies for CNS diseases.

Fig.1 Summary of some mechanisms of neuronal death. (Fricker, et al., 2018)

What Can We Do About Neuronal Death?

With our advanced platform and dedicated scientists, Creative Biolabs focuses on providing complete solutions for developing in vitro neurodegenerative disease models to customers around the world. Our professional scientists are experienced in offering tailored and one-stop neuronal death assays to advance the neurology studies of customers all over the world, which include but are not limited to:

• Neural cell culture

The culture of neural cells is fundamental to elucidating the cellular and molecular mechanisms underlying excitotoxic neuronal injury.

• Develop diverse CNS diseases models

The lack of access to physiologically relevant cellular models has greatly hampered the progress in CNS diseases. Creative Biolabs has successfully constructed a variety of different in vitro CNS disease models relying on the combination of iPSC technology and gene editing technology.

• Technologies for neuronal death assay

In addition to the traditional MTT colorimetric, trypan blue exclusion assay, and lactate dehydrogenase (LDH) test to determine neuronal death, Creative Biolabs quantifies neuronal cell death in different disease models by specific fluorescent cell death markers. Notably, a new method combining neuron-specific labeling with enzyme-linked fluorescence assays is also available at Creative Biolabs, which is readily adaptable for high-throughput in vitro screening of neurodegeneration.

• Screening campaigns

Creative Biolabs has successfully developed multiple unique neurodegenerative disease models, based on which we can optimize our available models to your specific needs or work with you to develop new in vitro models to aid in compound or drug research for CNS diseases.

Please contact us in time for more information and a detailed quotation.

Reference

1. Fricker, M.; et al. Neuronal cell death. Physiological reviews. 2018, 98(2): 813-880.