Schwann Cell Assay

Schwann cells are a type of glial cell that are essential for the proper functioning of the peripheral nervous system. They play a vital role in nerve regeneration and repair. Schwann cell assays are used to study the function and behavior of these cells. Creative Biolabs provides a comprehensive Schwann cell assay service that includes Schwann cell culture and characterization.

Schwann Cell Isolation and Culture

Our Schwann Cell Isolation service involves the isolation of highly purified schwann cells from various sources such as rat, mouse, and human peripheral nerve tissues. Our experts use optimized methods to isolate the cells while maintaining their viability and purity. The process involves enzymatic digestion of the nerve tissue followed by differential centrifugation, which results in the isolation of highly purified Schwann cells.

The culture of Schwann cells is a critical step in studying their function and behavior. Creative Biolabs uses advanced techniques to culture primary Schwann cells from rat, mouse, and human sources. The culture is maintained in a suitable environment with the necessary nutrients, growth factors, and hormones. Our team of experts ensures that the culture is free from contamination, and the cells are of high quality.

Figure 1. Nerve-derived SC cultures. (Monje, 2020)

Schwann Cell Characterization

Schwann cell characterization is a crucial step in understanding their function and behavior. Creative Biolabs provides a range of assays to characterize Schwann cells. These assays include immunofluorescence staining, gene expression analysis, and electrophysiology. Our team of experts uses state-of-the-art equipment to ensure accurate and reliable results.

• Immunofluorescence Staining

Immunofluorescence staining is a technique used to detect and localize specific proteins in cells. Creative Biolabs offers immunofluorescence staining to detect Schwann cell-specific markers such as S100 and P0. The staining provides information about the distribution and localization of these proteins in the cells.

• Gene Expression Analysis

Gene expression analysis is a technique used to study the genes that are active in a cell. Creative Biolabs offers gene expression analysis to identify the genes that are active in Schwann cells. This analysis provides information about the molecular mechanisms that underlie the function of these cells.

• Electrophysiology

Electrophysiology is a technique used to measure the electrical properties of cells. Creative Biolabs offers electrophysiology to measure the electrical properties of Schwann cells. This analysis provides information about the function of these cells in the peripheral nervous system.

Fig.2 Characterization of primary cultured Schwann cells (SCs). (Tan, 2018)

Our Advantages

Our team of experts has extensive experience in the field of neuroscience and has a deep understanding of Schwann cell biology. We use advanced techniques and state-of-the-art equipment to ensure accurate and reliable results. Our service offers several advantages, including:

In conclusion, Creative Biolabs provides a comprehensive Schwann cell assay service that includes Schwann cell culture and characterization. Our team of experts has extensive experience in the field of neuroscience and uses advanced techniques and state-of-the-art equipment to ensure accurate and reliable.

Get in touch with us today to find out more about our SC assay services.

References

1. Monje, Paula V. "Schwann cell cultures: biology, technology and therapeutics." Cells 9.8 (2020): 1848.
2. Tan, Dandan, et al. "Inhibition of RhoA-subfamily GTPases suppresses Schwann cell proliferation through regulating AKT pathway rather than ROCK pathway." Frontiers in cellular neuroscience 12 (2018): 437