Neuronal Microelectrode Array (MEA) Assay Service

Microelectrode arrays (MEAs) are a valuable tool for recording and measuring neuronal activity. This technology allows us to evaluate in real time how neuronal activity behaves in an environmentally controlled environment. In addition to spontaneous neuronal activity, electrical or compound stimulation can be performed, and over 40 different parameters are recorded simultaneously. To learn more about our products and services, submit a project inquiry, or request pricing, please contact us.

Introduction

Applications

• Electrical Activity in Cellular Disease Models
• Neuronal activity recording
• Drug Screening and Toxicity Testing
• Neural Network Modeling and Functional Analysis
• High-Density Recording and Signal Analysis

Case study: In Vitro Rat Spinal Cord Neuronal Activity

Primary rat spinal cultures were prepared and maintained in culture media for a minimum of 7 days, followed by treatment with excitatory neurotransmitters, an NMDAR inhibitor, a GABAA antagonist, and a K+ channel blocker.

Fig.1: Primary rat spinal cord neurons are harvested and plated on pre-coated MEA plates. Baseline neuronal activity is measured after min. 7 days in culture, followed by treatment of cells with compounds of interest. Changes in neuronal electrical activity can be evaluated after recording the MEA plate and analysis with the Axion Biosystems Axis and Neural Metric tools.

Fig.2 Primary rat spinal cultures were prepared and maintained in culture media for a minimum of 7 days, followed by treatments as indicated in the experimental scheme (Fig.1). A) Representative image of the firing pattern of the neurons during a recording. B-C) MEA recordings were carried out a regular intervals and different parameters such as mean firing rate and number of active electrodes increased over time in culture. D-F) Treatment of the neurons with excitatory neurotransmitter, NMDAR inhibitor, GABAA antagonist, and K+ channel blocker can differentially affect neuronal activity.

MEA technology has demonstrated significant potential and advantages in neuroscience research, offering high throughput, high resolution, versatility, and real-time monitoring capabilities. This makes it an invaluable tool for drug development, disease model construction, and neural network function research. We kindly request that you contact us to discuss your project plan in detail.