Product Overview

Description

The nuclear receptor subfamily 5 group A member 2, also known as NR5A2, is a 54 kD nuclear receptor protein. NR5A2 is a member of the FTZ-F1 sub-family and is highly expressed in the pancreas and to a lesser extent in the liver. NR5A2 contains a DNA binding domain, a hormone ligand-binding domain, and two zinc finger domains. It also binds to DNA as a monomer. NR5A2 is known to regulate the transcription of the liver enzyme cholesterol 7-alpha-hydrolase, which converts cholesterol to bile acids and also activates the hepatitis B virus enhancer II to contribute to the liver-specific expression of this virus. NR5A2 has been suggested to regulate pancreas-specific gene expression and to play a role in embryonic development. There are at least 3 alternatively spliced isoforms of NR5A2: isoforms 1 and 2 are present in fetal and adult liver and in liver cancer cells HepG2 as well.

Immunogen

Partial human NR5A2 recombinant protein (1-200 a.a.) expressed in E. coli.

Species Reactivity

Human

Clonality

Monoclonal

Host Species

Mouse

Isotype

IgG1, κ

Clone Number

CBP2201

Applications

Application Notes

WB (1.0-2.5 µg/mL)

Relevant Diseases

Brain Tumors; Alzheimer's Disease; Parkinson's Disease; Neurocutaneous Syndromes

Research Areas

Neuroinflammation

Conjugation

Unconjugated

Product Properties

Form

Liquid

Formulation

PBS, pH 7.2, containing 0.09% sodium azide.

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Purity

>95%, as determined by Coomassie stained SDS-PAGE.

Shipping

The product is shipped at 4°C. Upon receipt, store it immediately at the recommended temperature.

Storage

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Handling Advice

Store at -20°C. Upon initial thawing, apportion into working aliquots and store at -20°C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Target

NR5A2

Official Name

NR5A2

Full Name

Liver receptor homolog-1

Alternative Names

NR5A2; B1F; B1F2; CPF; FTF; FTZ-F1; FTZ-F1beta; LRH-1; LRH1; hB1F-2; nuclear receptor subfamily 5 group A member 2

Gene ID

2494 (Human); 26424 (Mouse)

Uniprot ID

O00482 (Human); P45448 (Mouse)

References

1. Li M, et al. 1998. J. Biol. Chem. 273:29022.
2. Nitta M, et al. 1999. Proc. Natl. Acad. Sci. USA 96:6660.
3. Lee YK, et al. 2002. J. Biol. Chem. 277:2463.
4. Zhang CK, et al. 2001. Gene 273:239.

Publications