Human Adrenal Neuroblastoma SiMa

Name: Human Adrenal Neuroblastoma SiMa
SKU: NCL2110P012
Rating: 5 (1 reviews)

[CAT#: NCL2110P012]

Species:: Human

Applications:: Cell Culture

Cell Types:: Neurons

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Product Overview

Features

Biotechnology: Used in a bioassay to measure botulinum toxin serotype A (BoNT/B) activity. Together with the monoclonal antibody produced by hybridoma 2E2A6 (CVCL_RA98).

Doubling time: 48-100 hours (PubMed=10686945); ~34-100 hours, highly variable (DSMZ).

Cell Types

Neurons

Applications

Cell Culture

Relevant Diseases

Neuroblastoma

Species

Human

Properties

Size

1x10^6

Form

Frozen

Culture Medium

DMEM high glucose (without sodium pyruvate) + 10% FBS + 1% P/S

STR Analysis

Amelogenin X,Y

CSF1PO 10,12

D2S1338 19,21

D3S1358 18

D5S818 11

D7S820 10

D8S1179 13,14

D13S317 12

D16S539 12,13

D18S51 14,16

D19S433 13,14

D21S11 28

FGA 20,22

Penta D 14

Penta E 11,12

TH01 9,9.3

TPOX 8

vWA 15,17

Biosafety Level

Cell Purity

>95%

Cell Viability

>90%

Mycoplasma Testing

The cell line has been screened using the luciferase based mycoplasma detection kit to confirm the absence of mycoplasma species.

Sterility Testing

Sterility testing was performed in accordance with USP and EP regulations. All of our sterility testing is performed in an isolator or clean room environments. The cell line has been screened using the membrane filtration testing methods to confirm the absence of aerobic, anaerobic and fungi microorganisms.

Genetic Stability Testing

Cell genetic stability study was perfomed under ICH guidelines. We provide guidance on the appropriate testing program upon your requirements.

Shipping

Dry ice

Storage

Liquid Nitrogen

Handling Advice

Frozen cells:Upon receipt, frozen ampoules should be transferred directly to gaseous phase liquid nitrogen without delay, unless they are to be used straight away.

Research Use Only

For research use only, not for diagnostic or therapeutic use.

Warnings

Store under recommended storage conditions (liquid nitrogen). Do not expose to high temperature. After expiration, discard all remaining reagents. It is recommended to use cells within ten generations.