Technology

Fluorescent Biosensor based Neuropeptide Imaging

Neuropeptides are small protein-like molecules that play crucial roles in neural communication and modulation. The ability to accurately detect and visualize neuropeptides in living systems is essential for understanding their dynamics and functions. Creative Biolabs proudly presents our cutting-edge service in Fluorescent Biosensor based Neuropeptide Imaging. This groundbreaking technology enables precise and real-time visualization of neuropeptides, unlocking new possibilities in neuroscience research.

Neuropeptides

Neuropeptides serve as critical mediators of neuronal communication and are involved in numerous physiological processes such as pain regulation, stress response, and appetite control. Understanding their distribution, release patterns, and interactions within the central nervous system is crucial for unraveling the complex mechanisms underlying brain function and dysfunction.

Table 1. Neuropeptides in the nervous and endocrine systems. (Hook, 2008)

Custom Fluorescent Biosensor Development

Creative Biolabs offers a comprehensive and customizable Fluorescent Biosensor Development Service specifically tailored for neuropeptide imaging. Our team of expert scientists leverages state-of-the-art technologies to engineer highly sensitive and specific biosensors capable of detecting and monitoring neuropeptide activity in real-time. By combining the power of fluorescence imaging with the precision of biosensor technology, our service empowers researchers with invaluable tools for unraveling the mysteries of neuropeptide signaling.

Our service encompasses the following steps:

Design and Construction of Biosensors

Creative Biolabs employs cutting-edge techniques in protein engineering and design to construct fluorescent biosensors with high affinity and selectivity for neuropeptides of interest. By strategically incorporating fluorescent reporter molecules into the biosensor framework, we ensure optimal signal generation and detection sensitivity.

In Vitro Characterization and Optimization

Before in vivo applications, our team rigorously characterizes and optimizes the performance of the biosensors in vitro. This involves assessing their binding kinetics, sensitivity, dynamic range, and specificity against a range of neuropeptide targets.

In Vivo Imaging

Creative Biolabs conducts in vivo imaging studies using animal models, allowing for the non-invasive monitoring of neuropeptide distribution, release, and kinetics in living systems.

Data Analysis

Our team of experienced bioinformaticians and neuroscientists assists in extracting meaningful insights from the imaging data, facilitating the understanding of neuropeptide signaling pathways and their role in neural circuitry.

Applications

By enabling the visualization and monitoring of neuropeptides in vivo, our biosensors offer unprecedented insights into neuropeptide signaling and its impact on brain function. Some key applications include:

1. Mapping Neuropeptide Release Patterns

Fluorescent biosensors allow researchers to map the spatiotemporal patterns of neuropeptide release in specific brain regions. By monitoring the fluorescence changes in real-time, researchers can visualize the dynamics of neuropeptide signaling during different physiological and pathological conditions. This information helps in identifying the brain regions and neural circuits involved in specific behaviors or disease states.

2. Studying Neuropeptide-Driven Behavior

Neuropeptides play a crucial role in regulating behavior. The custom Fluorescent Biosensors developed by Creative Biolabs enable researchers to investigate the relationship between neuropeptide release and behavioral responses. By imaging neuropeptide dynamics in freely behaving animals, researchers can decipher the role of specific neuropeptides in social interactions, feeding behavior, aggression, and other complex behaviors.

3. Evaluating Neuropeptide Therapeutics

Neuropeptides hold great therapeutic potential for various neurological and psychiatric disorders. By utilizing Fluorescent Biosensors, researchers can assess the effectiveness of neuropeptide-based therapeutics in real-time. The biosensors allow for the visualization of neuropeptide receptors.

Creative Biolabs also provide brain microdialysis service with real-time measurements of neuropeptides, allowing researchers to monitor neuropeptide release over extended periods. We offer comprehensive services in the development and optimization of custom microdialysis probes and the analysis of dialysate samples.

Please feel free to contact us for more about our animal models related services.

Reference

Hook, Vivian V. H. et al. "Proteases for processing proneuropeptides into peptide neurotransmitters and hormones." Annual review of pharmacology and toxicology 48 (2008): 393-423.

For Research Use Only. Not For Clinical Use.