Preformed Fibril (PFF) Model Development Service

Parkinson's disease (PD) is a debilitating neurodegenerative disorder characterized by the progressive loss of dopaminergic neurons and the accumulation of α-synuclein aggregates. PFF models have emerged as a powerful tool for recapitulating these key pathological features in experimental systems, providing valuable insights into disease mechanisms and facilitating the development of novel therapeutics. These models build upon decades of research into the role of α-synuclein in PD, as detailed in numerous publications. Creative Biolabs' Preformed Fibril (PFF) Models for PD help you accelerate your research and obtain more physiologically relevant data through our advanced PFF generation and characterization expertise.

How PFF Models Can Assist Your Project?

Creative Biolabs' PFF models provide researchers with a robust and reliable platform to investigate the pathogenesis of Parkinson's disease and evaluate potential therapeutic interventions. Our PFF models deliver consistent, reproducible results, enabling you to generate high-quality data and advance your research objectives.

• Deliverables, Solutions, and Key Problem-Solving Capabilities:
  • Accurate Disease Modeling: CBL's PFF models closely mimic the key pathological features of PD, including α-synuclein aggregation and propagation, allowing for more translational research.
  • Customizable Solutions: We offer tailored PFF model systems to meet your specific research needs, including variations in PFF type, injection site, and animal model.
  • Comprehensive Data Packages: Clients receive detailed reports, imaging data, and quantitative analyses, providing a complete understanding of the model's characteristics and treatment effects.

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Workflow:

• Required Starting Materials:

To initiate a PFF model study, clients typically provide the following:

1. Research Objectives: A clear outline of the study's goals, including specific hypotheses to be tested and desired endpoints.
2. Test Compounds (if applicable): Any therapeutic agents or compounds that will be evaluated in the PFF model.
3. Specific Animal Model Requirements: Details regarding the preferred animal species (e.g., mouse, rat), strain, and any specific genetic modifications.

• Final Deliverables:

Clients receive a comprehensive study report containing:

1. Detailed Methodology: A complete description of the experimental procedures, including PFF preparation, animal handling, injection parameters, and assessment methods.
2. Quantitative Data: Tabulated and graphical presentation of all experimental data, including behavioral scores, biochemical measurements, and histological quantification.
3. Statistical Analysis: A thorough statistical analysis of the data, including p-values, confidence intervals, and effect sizes.

• Estimated Timeframe:

The typical timeframe for a PFF model study at CBL ranges from 8 to 24 weeks, depending on the specific study design, animal model, and endpoints. Factors that may influence the duration include:

1. The complexity of the study design.
2. The time course of PFF-induced pathology in the chosen animal model.
3. The number of experimental groups and treatment conditions.
4. The specific behavioral and pathological assessments required.

Preformed Fibril (PFF) Models

Fig.1 Validation of α-syn pathology in the CNS and peripheral gut tissue of C57BL/6 J mice. Stereotaxic injection of 5 μg human PFF α-syn or monomer α-syn into striatum (AP+0.3, ML+2.3, DV−3.5) of C57BL/6 mice was performed.¹

Customer Reviews

"Using Creative Biolabs' PFF models in our research has significantly improved the translatability of our preclinical findings, allowing us to more accurately predict clinical outcomes." - Dr. A.*, Neurobiology

"Creative Biolabs' PFF model service provided us with highly reproducible data, which was crucial for our drug development program. The level of support and expertise was exceptional." - Prof. B.*, Pharmacology

"The detailed characterization of the PFF-induced pathology in Creative Biolabs' models has greatly facilitated our understanding of disease mechanisms and helped us identify potential biomarkers." - Dr. C.*, Neuroscience

What We Can Offer

Creative Biolabs offers a comprehensive suite of services to support your PFF model research, including:

• One-stop service: From laboratory-scale PFF production to large-scale animal studies.
• Efficient process development: Streamlined upstream and downstream process development for PFF generation.
• Large-scale capacity: Access to large-scale animal housing facilities and infrastructure to support studies of any size and scope.
• Quality system: Well-established quality system, Quality-by-Design (QbD), and process analytical techniques (PAT) to ensure the highest data integrity.
• Aseptic procedures: Strict aseptic verification procedures throughout the PFF injection and study process.
• Sustainable practices: Investment in environmental protection measures and skills.
• Documentation and validation: Documentation quality and procedures of PFF origin are assessed and approved by our quality assurance service.
• Strain stability: We guarantee the stability of PFF strains throughout the study, including any necessary pre- культивирования steps.
• Codon optimization: Optimization of the codon usage of genes to facilitate expression in selected animal models.
• Flexible study design: Ability to conduct studies using various administration and monitoring paradigms, including batch, fed-batch, or continuous modes.
• Culture optimization: Optimization of culture conditions to maximize the relevance and efficacy of the PFF model.
• Quality control: Implementation of high-standard quality control tools are used to quantify and evaluate the quality of PFFs and study results.

Related Services

Creative Biolabs offers a comprehensive portfolio of services to support your PD research, including:

• Custom Antibody Development: We provide tailored antibody solutions for the detection and characterization of α-synuclein and other relevant targets.
• Cell-Based Assays: Our cell-based assays can be used to study the effects of PFFs on neuronal function and survival.
• Histopathology Services: We offer expert histopathology services for detailed analysis of brain tissue from PFF model studies.

FAQs

Reference

1. Earls, Rachael H., et al. "Intrastriatal injection of preformed alpha-synuclein fibrils alters central and peripheral immune cell profiles in non-transgenic mice." Journal of Neuroinflammation 16 (2019): 1-15. Distributed under Open Access license CC BY 4.0, without modification.